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Complete Chloroplast Genome of
Clematis ranunculoides
:Characterization and Phylogeny Analysis
WANG Ruolan, ZHANG Dequan
Journal of Jishou University(Natural Sciences Edition) 2024, 45 (
1
): 66-76. DOI:
10.13438/j.cnki.jdzk.2024.01.012
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To reveal the chloroplast genome characteristics of Clematis ranunculoides and its phylogenetic relationships,the present study adopted the Illumina platform to acquire high-throughput sequences,which were used for assemble,annotate,and characterize the complete chloroplast genome of C. ranunculoides.And then molecular phylogenetic trees were constructed with maximum parsimony (MP),maximum likelihood (ML) and Bayesian (BI) methods.The result shows that the total length of the chloroplast genome of C. ranunculoides is 159 741 bp,which encodes 112 genes,including 79 protein-coding genes,29 tRNA genes and 4 rRNA genes;there are 84 SSRs in the chloroplast genome of C. ranunculoides,and 57 of them are mono-nucleotide.Analysis of codon usage bias reveales that leucine is the most frequently utilized amino acid in the chloroplast genome.The topological structures are basically identical among MP,ML,and BI trees.Derived from the phylogenetic trees,Clematis is a monophyletic group,closely related to Anemone.And C. ranunculoides is closest to C. montana,which supportes the traditional classification results.
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Research Progress of Structure and Function of Nuclear Transcription Factor Relish
ZHANG Hong, LIU Qingzhen
Journal of Jishou University(Natural Sciences Edition) 2024, 45 (
1
): 77-83. DOI:
10.13438/j.cnki.jdzk.2024.01.013
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The nuclear transcription factor Relish is a member of the NF-κbs superfamily functioning as an important immune signaling effector.Relish contains a conserved Rel homologue domain at the N-terminal and an ankyrin repeat domain at the C-terminal.Relish is actived in response to certain external stimulations transmitted by the Imd pathway,via phosphorylation,cleavage and other posttranslational modification of Relish.Activated Relish then translocates to nuclear and binds to DNA to regulate the expression of target genes,helping organisms to adapt to various physiological environments and stimuli,resisting the invasion and infection of foreign pathogens.The paper provides an brief review of the structure and function of Relish.The structural features of Relish include Rel homology domain,ankyrin repeats domain,nuclear localization sequence,caspase target site,serine-rich regions,and proline-glutamate-serine-rich domain.Functions of Relish mainly involve participating in innate immunity,regulating neural development and lifespan,regulating intestinal homeostasis and morphology,participating in stress regulation and other physiological activities.
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Identification of Cellulose Degrading Bacteria in Namco Yak Dung and Its Enzyme Production Characteristics
LI Qiang, LUO Jin, YANG Jianing, GAO Hongtao, LIU Xuan, HE Jianwu
Journal of Jishou University(Natural Sciences Edition) 2023, 44 (
3
): 61-65. DOI:
10.13438/j.cnki.jdzk.2023.03.009
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Through enrichment and purification,high efficient cellulose degrading strains were screened from yak dung in Namco Lake,Tibet.The enzyme production characteristics of cellulose degrading strains under different conditions were determined,and the genetic differences of the target strains were analyzed.The results showed that strain X1 and X2 had significant cellulose degradation advantage,and the relative enzyme activities of the two strains were strong at pH 4 and 9 ℃.Phylogenetic analysis showed that X1 and X2 might belong to the genus Cronobacter.
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Resistance to Kiwifruit Bacterial Canker and Its Correlation with Sugar Content in Branches of Kiwifruit Varieties
GAO Jianyou, KUANG Meimei, LUO Qin, TAN Zecheng, CUI Lihong, ZHA Manrong, LIU Shibiao
Journal of Jishou University(Natural Sciences Edition) 2023, 44 (
3
): 66-72. DOI:
10.13438/j.cnki.jdzk.2023.03.010
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In the present experiment,the resistance identification and evaluation of total 13 kiwifruit cultivars in Actinidia chinensis and A. chinensis var. deliciosa to Pseudomonas syringae pv. actinidiae (Psa) was conducted indoors by using wounding method on detached branches.The results showed that the incidence rate,onset time and lesion length of the isolated branches in all tested materials were significantly different after pathogenic bacteria inoculation.The order of resistance of the above cultivars was listed as follows:Miliang-1>Miliang-2>Chuhong>Cuiyu>Beimu>Xiangbiyu>Xiangji>Xiangjihong>Wuzhi-3>Jinyan>Donghong>Guichang>Hongyang,according to the disease lesion length from small to large,at the 25 d after infected Psa.A. chinensis var. deliciosa Miliang-1 was evaluated as the high resistance (HR) cultivar and A. chinensis Hongyang as the high susceptibility (HS) cultivar.The content of soluble sugar in branches decreased significantly after infected Psa,and the higher of the soluble sugar content after infection,the stronger resistance the cultivar had.However,no correlation was observed between the Psa resistance and reducing sugar content,lenticels characters and cortex thickness,respectively.
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Effects of Different Treatment on Seed Germination of Endangered Species
Terminalia myriocapra
YU Xiao, ZHAO Zhenning, DENG Lilan
Journal of Jishou University(Natural Sciences Edition) 2023, 44 (
3
): 73-79. DOI:
10.13438/j.cnki.jdzk.2023.03.011
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In this study,the effects of different exogenous hormones (GA3,NAA) and culture temperature on germination of the seeds were studied after hibernation treatment and indoor temperature presermation.The results showed that the type and concentration of growth regulator had significant effects (
P
<0.01),but the preservation method and culture temperature had no significant effects on the germination rate of seeds (
P
>0.05).The degree of influence was the type of growth regulator,the concentration of growth regulator,culture temperature and preservation method in descending order.After the seeds were treated with the same mass concentration of GA3 and NAA growth regulator,the germination rate of seeds was successively from high to low in variable temperature culture in hibernation treatment,constant temperature culture in hibernation treatment,constant temperature culture under normal temperature preservation,and constant temperature culture under normal temperature preservation.The optimal conditions for seed germination were treated at 4 ℃ for 30 days after seed collection,impregnated with 40 mg/L GA3 and cultured at 15~25 ℃ in a light incubator.
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Antibacterial Activity and Identification of Endophytic Fungi YDZ-8 Isolated from
Lavandula angustifolia
Mill.
CHEN Tianyi, BAIDOULA·Nulan, TUERDEBIEKE·Mayila, ENTOMACKY·Borrathyba
Journal of Jishou University(Natural Sciences Edition) 2023, 44 (
2
): 47-51. DOI:
10.13438/j.cnki.jdzk.2023.02.007
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The endophytic fungi were isolated from the roots of
Lavandula angustifolia
Mill. by surface disinfection method and tissue isolation method,and the antibacterial activity against the Staphylococcus aureus was evaluated by paper disk diffusion method.The active isolates were identified in terms of their morphological features by phylogenetic analysis based on ITS sequence of rDNA gene.The results showed that the endophytic fungi of YDZ-8 had remarkable antibacterial activity against Staphylococcus aureus strain CGMCC1.872.Fungal morphological and molecular identification demonstrated that the endophytic fungi strain YDZ-8 represented Trichoderma harzianum.
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Analysis of the Rhizosphere Soil Bacterial Community Composition of Kiwifruits with Canker Disease and Healthy Kiwifruits
YANG Rui, YAO Di, TANG Yunzhe, ZHANG Jingyi, PENG Qingzhong, WANG Linluosha
Journal of Jishou University(Natural Sciences Edition) 2023, 44 (
1
): 59-67. DOI:
10.13438/j.cnki.jdzk.2023.01.009
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In this work,the rhizosphere soil samples at three stages of kiwifruit development were collected from the kiwifruit planting base in Yongshun County,West Hunan.The bacterial community composition and population abundance in the canker and healthy samples were analyzed by metagenomic sequencing.The results showed that there were some differences in the bacterial community composition and population abundance in three periods of rhizosphere soil samples of the healthy and diseased kiwifruit,but the dominant bacteria mainly included AcidobacteriaGp,Gemmatimonas and Gaiella.The population abundance of Pseudomonas increased and then decreased with three stages of kiwifruit development,coinciding with the process of kiwifruit canker disease,which indicates that the rhizosphere soil bacterial community is one of the infectious sources for the kiwifruit canker occurrence.In addition,it was found the abundance of flavobacteriaceae and comamonadaceae was higher in the canker samples than in the healthy samples,presumably positively correlated with the occurrence of kiwifruit canker disease.The abundances of Rhodospirillaceae,Thermomonosporaceae,Bacillus and Streptomyces were higher in the healthy samples than in the diseased samples,presumably playing an antagonistic role in the occurrence of kiwifruit canker disease.
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Hemiboea gracilis
Franch. var.
guixiensis
: A New Variety of Gesneriaceae from Hunan,China
TAN Lu, XIANG Xiaomei, LIU Bing, CHEN Gongxi
Journal of Jishou University(Natural Sciences Edition) 2021, 42 (
2
): 61-64. DOI:
10.13438/j.cnki.jdzk.2021.02.011
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A new variety of Gesneriaceae,
Hemiboea gracilis
Franch. var.
guixiensis
G. X. Chen, X.M.Xiang et L. T var. nov. from Hunan Province of China is described. The new variety is similar to the typical variety (
Hemiboea gracilis
Franch.) in morphology. But further observation showed that the new varietystems is sparsely pilose, leaf sides and petiole are densely bristly, except the inside of the flower, the rest is densely pilose, the fruit tips are sparsely pilose. Therefore, they are easy to distinguish.
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Classification Standard for One-Year Seedling of
Phellodendron chinense
LIU Nianyuan, ZHONG Qingshan, XIANG Zuheng, PENG Xianfeng, ZENG Huijie, XIANG Hua, XIANG Ming, XIANG Caihai, HUANG Zhengming
Journal of Jishou University(Natural Sciences Edition) 2021, 42 (
2
): 65-71. DOI:
10.13438/j.cnki.jdzk.2021.02.012
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Aiming to instruct the field management and to push the normalization and standardization of
Phellodendron chinense
Schneid seedling cultivation, the critical values for classification of 1 year’s old
Phellodendron chinense
Schneid seedlings were determined using materials of 2 564 plants in 72 sample plots with 1 m×1 m size in Dan township, Longshan county, Hunan province according to quality indexes of ground diameter (D) and height (H) of seedling with K-average value clustering method and standard deviation method. The comprehensive evaluation result was as fellows, the standard for class Ⅰ seedling was D≥1 cm and seedling H≥100 cm, and class Ⅱ seedling was 0.6~<1 cm for D, and 60~<100 cm for seedling H, and class Ⅲ seedling was D<0.6 cm and seedling H<60 cm. According to this standard, the proportion of class Ⅰ and class Ⅱ of Phellodendron chinense is 64.8%.
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Investigation and Exploitation of Edible Medicinal Plant Resources in Xiangxi region, China
CHEN Jiabei, WANG Lu, LIU Jinyuan, DENG Shanshan, HU Die, CHEN Gongxi
Journal of Jishou University(Natural Sciences Edition) 2021, 42 (
1
): 66-77. DOI:
10.13438/j.cnki.jdzk.2021.01.011
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In order to understand the current situation of medicinal and edible wild plants resources and development and utilization in Xiangxi region. By means of field investigation and literature review, the family composition, living habits, habitat and distribution pattern, function and utilization of medicinal and edible plants in this area were investigated and analyzed. The results showed that there were 57species of medicinal and edible wild plants belonging to 30 families and 52 genera in Xiangxi region. Among them, Liliaceae, Compositae, Gramineae, Lamiaceae and Rosaceae were the dominant quantitative families, Polygonatum, Lilium, Amygdalus and Ziziphus were the dominant quantitative genera. There are 35 herbaceous plants and 23 woody plants, and herbaceous plants are dominant. Among 21 habitats, hillside, cultivated land, forest and roadside are important habitats for medicinal and edible plants in Xiangxi region. In the distribution pattern, the horizontal distribution pattern of the southern and northern regions is basically uniform. The vertical distribution was significantly different, and the medicinal and edible plants were most distributed in the low mountain area. In terms of function and utilization, there are many functions, parts and methods of medicinal and edible plants. Among them, plants with heat-clearing medicinal and edible health care effect are the most. Plants with edible and medicinal stems accounted for the highest proportion. Most plants are decocted for medicine and cooked in soup/porridge. Xiangxi region is rich in medicinal and edible plant resources and has great potential. The protection, comprehensive research, development and utilization of local medicinal and edible plants should be carried out, and corresponding measures should be taken to promote the development of medicinal and edible plant resources in Xiangxi region.
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Researches and Prospect on Plant Diversity of Dehang Scenic Spot in Hunan Province, China
XIANG Xiaomei, TAN Lu, LIU Bing, CHEN Gongxi
Journal of Jishou University(Natural Sciences Edition) 2020, 41 (
5
): 45-51. DOI:
10.13438/j.cnki.jdzk.2020.05.010
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This paper reviewes the plant investigation, floristic study, relationship between plants and the special canyon eco-environment, and research status quo of of plant resources utilization of plant diversity in Dehang scenic spot. This review analyzes the unsolved scientific problems and looks forward to the future research. In the future, we should focus on the investigation of plant diveristy and the adaptive mechanisms of plants in particular canyon eco-environment, strengthen the management and construction of plant resources and the park, to achieve the protection of plant diversity and sustainable developlment of resources in Dehang scenic spot.
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Analysis on Volatile Components in Roots of
Cyclocodon lancifolius
(Roxburgh) Kurz by GC-MS Combined with Retention Index
WANG Jianxia, LIU Yihan, TIAN Yungang, GUO Hongwei, LI Ming, BI Shaowen, WEI Hua
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2020.04.011
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A New Blind Subterranean Species of the Genus
Bibarba
from Yunnan, China
YANG Hongfu, CHEN Ziming, LI Weixian
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2020.01.012
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Community Characteristics of Reevesia
Pubescens Xuefengensis
in Gaowangjie National Nature Reserve, Hunan Province
WANG Benzhong, LIU Huihua, YANG Sen, PENG Jingbing, XIANG Shijun
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2020.01.013
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A New Species of Cyprinus from Yunnan,China:
Cyprinus (Cyprinus) Bajiangensis
LI Weixian
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2019.02.009
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Analysis and Evaluation of Nutritive Composition of the Leaves from
Trichosanthes Kirilowii
Maxim
WU Shuailin,CHEN Ya,LI Gui,CHEN Gongxi,XIONG Lizhi
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2019.02.010
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A New Species of
Sinocyclocheilus
from Yunnan,China:
Sinocyclocheilus
Bannaensis
LI Weixian,LI Chunqing,CHEN Hongyu
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2019.01.015
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Content Measurement of Oleanolic Acid in
Akebia Trifoliata
Pericarp by Ultraviolet-Visible Spectrophotometry
FU Zhirong,LI Xuefeng,ZHANG Xiaoxu,OUYANG Yuzhu,WU Mengyuan
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2019.01.016
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Bioinformatics Analysis of
Trichoderma Reesei
Biodegrading Enzyme CIP 1 and CIP 2
ZHANG Hong,WEI Xueying,CHEN Jiabei,CHEN Sijia,HE Jianwu
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2018.05.011
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Effects of Gymnocladas Saponin DNA Damage and Serum Cholesterol in Rats
LI Yubai,HUANG Hongyan,LI Fang
Journal of Jishou University(Natural Sciences Edition) DOI:
10.13438/j.cnki.jdzk.2018.05.012
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Chemical Analysis of Seed Oil from Abelmoschus Manihot by GC-MS
ZHU Manxiang,ZHENG Jie,WANG Jianxia,LIU Yihan,TIAN Yungang,GUO Hongwei,WEI Hua,
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2018.03.011
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Two New Species of
Sinocyclocheilus
from Guangxi,China
WU Zhiluan,LI Chunqing,LAN Chun,LI Weixian
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2018.03.012
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Optimization of Ultrasonic-Assisted Extraction of Flavonoids from
Artemisia Princeps
Pamp. by Response Surface Methodology
ZHANG Shan,XIAO Juan,SONG Xiaohui,XIONG Lizhi
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2018.02.013
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Genetic Diversity of mtDNA D-Loop Sequences in Wujin Pig
WANG Zhimin,XU Yaou,SUN Yan,YANG Lei,XU Longyang, WANG Yingming,HUANG Weiping
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2018.02.014
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Screening of a Methylotrophic Bacteria
Serratia Marcescens
NH8 and Isolation of Its Pyrroloquinoline Quinone (
pqq
) Genes Cluster
PENG Qiaomu,ZHOU Lulu,XIONG Li,PENG Qingzhong,HU Guang
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2018.01.013
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Species of Freshwater Mussels in Anhui Province
ZHAN Xiaodong,WANG Shaosheng,HUANG Yue'e,TAO Ning,LI Chaopin
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2018.01.014
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Antimicrobial Activity of Aconitine-Producing Endophytic Fungus from Aconitum Leucostomum Worosch and its Identification
TUERDEBIEKE Mayila,NAZIERBIEKE Wulumuhan,ZHANG Na,BORRATHYBAY Entomack
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.06.012
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A New Genus and Species of Labeoninae from Yunnan China
YANG Hongfu,LI chunqing,CHEN Hongyu,LI Weixian
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.06.013
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Comparison of BIM Among Chinese Han,Japanese and Korean
ZHENG Lianbin,YU Keli,LI Yonglan,XI Huanjiu,BAO Jinping,ZHANG Xinghua
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.04.013
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Microsporogenesis and Development of Male Gametophyte in
Cinnamomum Paueiflorurn
Nees
LONG Hua
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.04.014
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Screening and Identification of a High-Temperature Resistant Cellulose-Degrading Bacterium
YI Min,YANG Yuting,LI Menglin,ZHANG Li,HE Jianwu,LIU Zhuxiang,CHEN Yiguang
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.04.015
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Phylogenetic Evolution of DELLA Gene Family in Angiosperms
HUA Nan,MENG Ying,CHU Zhaofu,NIE Zelong
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.03.010
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Effects of Auxin and Its Transport Inhibitors on Spore Germination in
Ceratopteris Pteridoides
PENG Yi,LIU Lu,LI Guisheng
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.03.011
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Extraction Conditions and Determination of Antibacterial Activity in Vitro of Konjac Glucomannan
XIONG Wuguo,LI Taibing,LI Jiaxing,DENG Chong,HUANG Cheng
Journal of Jishou University(Natural Sciences Edition) DOI:
10.3969/j.cnki.jdxb.2017.03.012
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