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25 November 2024, Volume 45 Issue 6

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Regional Medicinal Resources

Research Status and Clinical Application of the Tujia Medicinal Swertia davidii

HE Tingxiang, FU Qingqing, ZHU Jiaxin, PENG Shengwen, LIU Fen

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 1-10.

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To further explore the medicinal potential of Tujia medicine,this paper comprehensively reviews the research progress of Swertia davidii in six aspects,including crude drug identification,chemical composition,content determination,extraction technology,pharmacological action,and clinical application,by referring to Chinese herbal medicine classics and related literature.The results indicate that Swertia davidii is abundant in various chemical components,including xanthones,iridoids,triterpenoids,and flavonoids,and exhibits diverse pharmacological activities such as anti-inflammatory,antibacterial,antitumor,antiviral,and antioxidant properties.In clinical practice,Swertia davidii is extensively used in the treatment of pulmonary fever,pneumonia,jaundice,cholecystitis,acute and chronic enteritis,gynecological inflammation,dysentery,diarrhea,urinary tract infections,scabies,oral ulcers,and other diseases,demonstrating significant medicinal value.

Effects of Volatile Oil of the Alpinia japonica Rhizome on Gastrointestinal Motility of Mice in Tujia Medicine

ZHAO Jing, XU Taijing, LUO Jing, PENG Qingbo, GAO Zhihong, XIANG Juan

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 11-15.

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To investigate the effects of volatile oil of the Alpinia japonica rhizome on the gastrointestinal motility of mice,mice were divided into control group,rhubarb group,volatile oil of the Alpinia japonica rhizome 5.2 g/kg dose group,and volatile oil of the Alpinia japonica rhizome 10.4 g/kg dose group.The effects of volatile oil of the Alpinia japonica rhizome on the intestinal propulsion function,defecation function,and gastric emptying function of mice were explored through intestinal propulsion test,defecation observation,and gastric emptying test.The results showed that the charcoal retention rate of the rhubarb group,volatile oil of the Alpinia japonica rhizome 5.2 g/kg dose group,and volatile oil of the Alpinia japonica rhizome 10.4 g/kg dose group was all higher than that of the control group;the first black defecation time of the rhubarb group,volatile oil of the Alpinia japonica rhizome 5.2 g/kg dose group,and volatile oil of the Alpinia japonica rhizome 10.4 g/kg dose group was all earlier than that of the control group;the gastric emptying rate of the rhubarb group,volatile oil of the Alpinia japonica rhizome 5.2 g/kg dose group,and volatile oil of the Alpinia japonica rhizome 10.4 g/kg dose group was all higher than that of the control group;the enhancement effect of volatile oil of the Alpinia japonica rhizome 10.4 g/kg dose group was comparable to that of the rhubarb group.The results suggest that volatile oil of the Alpinia japonica rhizome has certain promoting effects on the intestinal propulsion,defecation,and gastric emptying of mice.

Resources and Characteristics of Folk Medicine for Bruises in Baojing County

LIU Hebing, SU Xiujiang, YANG Dequan, SHE Yonghong

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 16-23.

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To sort out Baojing County's resources of ethnic medicines for the treatment of bruising injuries,in terms of plant sources,names,properties,efficacy and clinical application,this article introduces 51 kinds of Tujia and Miao ethnic medicines commonly used in Baojing County,and offers suggestions for how to use them to protect and sustain ethnic medicine resources.

Medicinal Chemistry and Pharmacology

Purification Process of Total Flavonoids from Lilium lancifolium Thunb and Its Antibacterial Activity

ZHU Lanping, YUAN Jiao, YUAN Jieying, YIN Xinlian, LIU Ying, CHENG Jiang, YAO Shufeng

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 24-30.

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Through comparing the static adsorption-desorption performance of 7 kinds of macroporous resins,the best macroporous resin with high adsorption rate and desorption rate was selected.The optimal purification process of Lilium lancifolium by macroporous resin was selected by single factor experiment and orthogonal experiment,and the total flavonoid content and antibacterial activity before and after purification were determined.The results showed that the macroporous resin with high adsorption and desorption performance was AB-8.The optimal purification process was as follows:the total flavonoid concentration of the feed solution was 0.076 4 g/L,the pH value was 4,the adsorption time was 8 hours,the solvent volume fraction was 60%,the solvent volume was 120 mL,and the desorption time was 10 hours.The total flavonoid content after purification was 11.55%,which was 5.3 times that of the purification before.The inhibition zone diameter of the purified total flavonoid was larger than that of the total flavonoid before purification,and the inhibitory effect on Staphylococcus aureus was higher than that on Escherichia coli.

Extraction Process and Antibacterial Activity of Total Saponins from Rhizomes of Rohdea chinensis

GUO Wei, MAO Jiayi, XU Liang, QING Dujiang, CHENG Jiang, YAO Shufeng

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 31-36.

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The total saponin was isolated from Rohdea chinensis rhizome through the ethanol water bath heating extraction method.The influences of ethanol concentration,liquid to material ratio,extraction time and extraction temperature on the yield rate of the total saponin were investigated.On the basis of single factor test,the orthogonal test was used to optimize the extraction process.The optimal extraction conditions of total saponins from the rhizome of Rohdea chinensis are as follows:material-liquid ratio 1∶30 (g∶mL),ethanol concentration 60%,extraction time 60 min,and extraction temperature 80 ℃.Under these extraction conditions,the total saponin yield of Rohdea chinensis was 17.46%.The results of the antibacterial activity test showed that the total saponins in the rhizome of the Rohdea chinensis had inhibitory effects on Escherichia coli,Staphylococcus aureus,Candida albicans,and the inhibitory activity on Staphylococcus aureus was the best,with the diameter of the inhibition circle 12 mm at a concentration of 5 g/L.

Optimization of Extraction Process and Vitro Antioxidant Activity of Total Polysaccharides from Polygonatum odoratum

ZHANG Yan, ZHANG Yang, CHEN Jiarui, YAO Shufeng, CHENG Jiang

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 37-43.

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The extraction process and antioxidant activity of total polysaccharide from the rhizome of Polygonatum odoratum were evaluated.The total polysaccharide was isolated from Polygonatum odoratum rhizome through ultrasound-assisted water bath heating extraction method.The influences of material-liquid ratio,ultrasonic power,ultrasonic extraction temperature on the extraction rate of the total polysaccharide were investigated.Based on the results of single-factor experiments,the response surface experiments give the optimal extraction conditions of total polysaccharide from the rhizome of Polygonatum odoratum∶ material-liquid ratio 1∶15 (g∶mL),ultrasonic power 300 W,extraction time 60 min,and ultrasonic extraction temperature 60 ℃.Under these extraction conditions,the total polysaccharide yield of Polygonatum odoratum was 13.58%.The results of the antioxidant activity test showed that when the mass concentration of Polygonatum odoratum polysaccharide was 2.75 g/L,the scavenging rates of Polygonatum odoratum polysaccharide on DPPH radicals and OH radicals were 34.18% and 62.04%,respectively.

Mechanism of Caulis Sinomenii in Treating Rheumatoid Arthritis Based on Network Pharmacology and Molecular Docking

WU Ziqi, GONG Bufan, WANG Jianchao, PENG Huayong

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 44-53.

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To study the mechanism of Caulis Sinomenii in the treatment of rheumatoid arthritis,network pharmacology and molecular docking were used to screen 6 main active ingredients of Caulis Sinomenii from the TCMSP database.268 drug targets corresponding to the active ingredients were obtained through the PubChem library and Swiss Target Prediction.Subsequently,5 706 targets for rheumatoid arthritis were obtained through GeneCards,DisGeNET,and OMIM databases.By utilizing the Venny 2.1.0 platform to draw Venn diagrams,178 intersection targets between the active ingredients of Caulis Sinomenii and rheumatoid arthritis diseases were identified.A network diagram illustrating the interaction between the active ingredients of Caulis Sinomenii and rheumatoid arthritis disease was constructed using Cytoscape 3.10.0.Additionally,a drug component target protein network diagram was created.GO enrichment analysis and KEGG pathway enrichment analysis were conducted using the DAVID database.Molecular docking simulation was performed between the target and the active ingredients of the drug using Autodock software.The results revealed that NR3C1,AKT1,SRC,PPARG,and JUN are the key target proteins for the anti-rheumatoid arthritis effect of Caulis Sinomenii.The GO enrichment analysis results indicated that biological processes mainly include G protein-coupled receptor signaling pathway,coupled to cyclic nucleotide second messenger G,vascular process in circulatory system,adenylate cyclase-modulating G protein-coupled receptor,peptidyl-serine phosphorylation,etc.The cellular components mainly include integral component of presynaptic membrane,intrinsic component of presynaptic membrane,membrane raft,membrane microdomain,etc.The molecular functions mainly involve nuclear receptor activity,ligand-activated transcription factor activity,catecholamine binding,protein tyrosine kinase activity,etc.According to the KEGG enriched bubble diagram,it can be inferred that the affected pathways include the cancer pathway,the neuroactive ligand receptor interaction pathway,and the inflammatory mediator regulatory pathway of the TRP channel.The molecular docking results indicated that the active ingredients,including β-sitosterol,razmanine,xylosterol,sinomenine,and levodontidine,are associated with the core targets NR3C1,AKT1,SRC,PPARG,and JUN,all showing good binding activity.The treatment of rheumatoid arthritis with Caulis Sinomenii may involve a multi-component,multi-target,and multi-pathway process,which includes regulating inflammatory cytokines,immune cells,cell cycles (such as apoptosis and burning),and osteoblasts and osteoclasts to treat rheumatoid arthritis.

Drug analysis and Identification

GC-MS Fingerprint of Petroleum Ether Fraction in Paulownia fortunei Leaves

LI Yuhong, GUO Hongwei, HUANG Jia, YANG Lili, LI Ming, LONG Guoqing, WEI Hua

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 54-61.

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To evaluate the quality of Paulownia fortunei leaves from different regions,gas chromatography-mass spectrometry was used to qualitatively analyze the petroleum ether fraction of Paulownia fortunei leaves.Squalene used as a control,the similarity evaluation system of traditional Chinese medicine chromatographic fingerprint (2012A version) used for similarity evaluation,cluster analysis and principal component analysis were combined to evaluate the quality.The results indicated that a total of 12 common peaks were identified in 19 batches of Paulownia fortunei leaves from various regions.These peaks corresponded to the following compounds:4,4,7a-Trimethyl-5,6,7,7a-tetrahydro-1-benzofuran-2(4H)-one,phytol,heptadecanoate,2-methyl octacosane,squalene,nonacosane,triacontane,hexatriacontane,α-Tocopherol,nonatriacontane,tetracontane,β-Sitosterol.Through cluster analysis,19 batches of Paulownia fortunei leaves were divided into 3 major categories.The cumulative variance contribution rate of the two principal components obtained from principal component analysis was 86.725%,and the results showed that sample S12 had a higher comprehensive score.The results of principal component analysis and cluster analysis were basically consistent.

Application of Simplified Thin-Layer Chromatography in the Analysis of Paulownia fortunei Leaves

LI Ming, WANG Qingrong, LI Yuhong, GUO Hongwei, LONG Guoqing, LYU Shuang, WEI Hua

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 62-67.

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A simplified one-dimensional thin-layer chromatography (TLC) method has been established for the identification of Paulownia species leaves,including Paulownia fortunei,Paulownia elongata,Paulownia tomentosa and Paulownia kawakamii.A single-factor experiment was conducted to select the optimal two-dimensional multiple development TLC method,which was then combined with column chromatography to segment the extract.Then,the one-dimensional TLC method was employed to identify the leaves of the four Paulownia species after developing the concentrated segments.The results showed that in the two-dimensional multiple expansion,characteristic spots of Paulownia fortunei were identified.Additionally,in the one-dimensional thin layer after segmented concentration,four common characteristic spots were observed for the four Paulownia species at 254 nm,seven common spots at 365 nm,and twelve common spots after color reaction under sunlight.The differences between Paulownia fortunei and the other three species were noted:on the developed TLC plate after color reaction,the first segment of the small polar sample showed a slightly yellow spot at an Rf value of 0.5,while the third segment of the large polar sample exhibited two light pink spots at an R_f value of approximately 0~0.2.

Thin-Layer Chromatography Identification of Paris fargesii Rhizome,Trillium tschonoskii Rhizome,and Paris Rhizome

YANG Dequan, SHE Yonghong

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 68-71.

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Paris Saponin II,VI,H were used as reference substances,and the rhizomes of Paris fargesii,Trillium tschonoskii,and Paris (The rhizome of Paris polyphylla Smith var. chinensis (Franch.) Hara and Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz.) were identified by thin-layer chromatography (TLC).The results showed that the three reference substances had high resolution and clear spots on the silica gel G thin-layer plate with chloroform-methanol-water (15∶5∶1) as the developing agent under the conditions of 20 ℃±2 ℃ and 50%~60%.According to the difference in components,TLC can be used to distinguish Paridis fargesii rhizome,Trillium tschonoskii rhizome,and Paris rhizome.

Technology Research and Application

Implications of Self-Healing for the Research and Development of New Drugs and New Treatment Technologies for Diseases

YANG Maosheng

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 72-76.

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In order to explore a new path for research and development of new drugs and new treatment technologies for diseases,literatures relating to self-healing from Google Scholar,PubMed database,and Websites was retrieved and analyzed.It can be found that many human diseases show the possibility of self-healing.According to the above findings,a novel insight into developing new drugs and new treatment technologies for diseases is proposed based on triggering self-healing.

Development of a Useful Compound Functional Beverage with Eucommia ulmoides-Vinegar

XIANG Xiaomei, FU Li, LI Xinyao, PENG Hongjin, HE Jianwu, CHEN Gongxi

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 77-83.

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The production process of the compound functional beverage of Eucommia Chinese black tea and rice vinegar was studied as the main raw materials.Design-Expert 8.0.6 was used to design the experiments and the sensory score of Eucommia ulmoides-vinegar used as the index to analyse the experimental data on the response surface.The results showed that the optimal formula of the Eucommia ulmoides-vinegar was the ratio of tea to water 4∶100（w∶w）,the addition amount of rice vinegar 2.28%,the addition amount of juice A 19.05%,and the addition amount of excipient B 7.76%.Under these conditions,the sensory score of Eucommia ulmoides-vinegar was the highest,reaching 88.4 points.

Establishment and Preliminary Application of Tail Suspension Analysis System Based on DeepLabCut

LI Zhihong, SHENG Yihua, GU Xinli, ZHOU Cenfei, WANG Huizhi, JIA Zhujun, ZHANG Jianzhi, LI Sidi

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 84-91.

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A tail suspension analysis system has been established based on DeepLabCut (DLC) algorithm for evaluating depressive behavior in mice.Based on the DLC algorithm in deep learning technology,multi-path,semi-closed tail suspension hardware,the systems of software and hardware were constructed;this system and open field analysis system were applied to evaluate the tail suspension and open field behavioral changes in mice with reserpine depression model,intermittent alcohol consumption injury model,and periodontal silk ligation model.This system realized the synchronous analysis of head movement and limb movement in tail suspension,and automatically quantified 50 tail suspension indexes of dynamic and static type,posture type,and intensity type.It was found that in the reserpine depression model,compared with the control group,the movement distance,wall climbing times,and head swing times were reduced in the low-,medium-,and high-dose reserpine groups in 2 days,and the head swing times were reduced in the medium- and high-dose reserpine groups in 3 days and 4 days.In the intermittent alcohol consumption injury model,compared with the control group,the immobility time was reduced,the limb struggling times,movement distance,and movement time were increased in the low- and medium-dose ethanol groups,and the forelimb,hindlimb,and absolute struggling times were reduced in the high-dose ethanol group.In the periodontal silk ligation model,the movement distance and length,limb and absolute struggling times,and the total value of forelimb,hindlimb,and limb angles were reduced in the ligation group.Clearly,the DLC algorithm-based mouse tail suspension analysis system can accurately distinguish the behavioral characteristics of different mechanistic depression models.

Transformative Efficacy of Albumin-Bound Paclitaxel Combined with Tegafur,Gimeracil and Oteracil for Unresectable Pancreatic Cancer

SONG Xin, ZHOU Guochao, YANG Dinghua, FU Hua, ZHOU Lyu, CAI Rongmin, TIAN Tanping

Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 92-96.

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In order to explore the efficacy and safety of albumin paclitaxel combined with tegafur (AS) regimen and albumin paclitaxel combined with gemcitabine (AG) regimen in the treatment of unresectable pancreatic cancer,patients with unresectable advanced pancreatic cancer who received AS regimen (24 cases) and AG regimen (16 cases) were selected as study subjects.Objective response rate,overall survival,progression-free survival and the incidence of grade 3/4 adverse reactions were compared between the two groups.The results showed that the objective response rate,median survival time and median progression-free survival time in AS group were higher than those in AG group,and there was no statistical significance between groups (P>0.05).The incidence of grade 3/4 peripheral nerve toxicity in AS group was significantly lower than that in AG group,and the difference was statistically significant (P<0.05).The incidence of grade 3/4 platelet count decrease,leukopenia,hemoglobin level decrease,infectious fever,nausea/vomiting,liver function impairment and poor appetite in AS group was lower than that in AG group,and the difference was not statistically significant (P>0.05).In the treatment of unresectable pancreatic cancer,the AS regimen has a lower risk of adverse effects.