Antibacterial Activity and Identification of Endophytic Fungi YDZ-8 Isolated from Lavandula angustifolia Mill.

doi:10.13438/j.cnki.jdzk.2023.02.007

Abstract

Abstract: The endophytic fungi were isolated from the roots of Lavandula angustifolia Mill. by surface disinfection method and tissue isolation method,and the antibacterial activity against the Staphylococcus aureus was evaluated by paper disk diffusion method.The active isolates were identified in terms of their morphological features by phylogenetic analysis based on ITS sequence of rDNA gene.The results showed that the endophytic fungi of YDZ-8 had remarkable antibacterial activity against Staphylococcus aureus strain CGMCC1.872.Fungal morphological and molecular identification demonstrated that the endophytic fungi strain YDZ-8 represented Trichoderma harzianum.

Key words: Lavandula angustifolia Mill., endophytic fungi, antibacterial activity, ITS sequences

CHEN Tianyi, BAIDOULA·Nulan, TUERDEBIEKE·Mayila, ENTOMACKY·Borrathyba. Antibacterial Activity and Identification of Endophytic Fungi YDZ-8 Isolated from Lavandula angustifolia Mill.[J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(2): 47-51.

References

[1] ANTONY STURZ,JERZY NOWAK.Endophytic Communities of Rhizobacteria and the Strategies Required to Create Yield Enhancing Associations with Corps[J].Applied Soil Ecology,2000,15(2):183-190.
[2] ANDERA STIERLE,GARY STROBLE,DONALD STIERLE,et al.Taxol and Taxane Production by Taxomyces Andreanae,an Endophytic Ungus of Pacific Yew[J].Science,1993,260(5105):214-216.
[3] 符继红,唐军,廖享,等.基于抑菌活性成分的薰衣草精油组效关系研究[J].质谱学报,2015,36(5):403-410.
[4] 姜黎,王向未,江英.薰衣草精油抑菌作用的研究[J].农产品加工,2011(4):73-75.
[5] 刘凡,李敏,顾爱星,等.薰衣草精油对几种植物病原菌的抑菌作用[J].新疆农业科学,2017,54(5):938-944.
[6] ALEXA ERSILIA,DANCIU CORINA,RADULOV ISIDORA,et al.Phytochemical Screening and Biological Activity of Mentha Piperita L. and Lavandula Angustifolia Mill. Extracts[J].Analytical Cellular Pathology,2018,2018:1-7.
[7] ZHAO Yunqi,CHEN Ran,WANG Yun,et al.In Vitro and in Vivo Efficacy Studies of Lavender Angustifolia Essential Oil and Its Active Constituents on the Proliferation of Human Prostate Cancer[J].Integrative Cancer Therapies,2017,16(2):215-226.
[8] MIASTKOWSKA MAGORZATA,KANTYKA TOMASZ,BIELECKA EWA,et al.Enhanced Biological Activity of a Novel Preparation of Lavandula Angustifolia Essential Oil[J].Molecules,2021,26(2458):21-40.
[9] BOGDAN MIHAELA,BUNGAU SIMONA,TIT DELIA,et al.Chemical Profile,Antioxidant Capacity,and Antimicrobial Activity of Essential Oils Extracted from Three Different Varieties (Moldoveanca 4,VisMagic 10,and Alba 7) of Lavandula Angustifolia[J].Molecules,2021,26(4381):20-39.
[10] MALEKIFARD FARNAZ,TAVASSOIL MOUSA,ALIMORADI MOHAMMAD.In Vitro Assessment of Anti-Trichomonas Effects of Zingiber Officinale and Lavandula Angustifolia Alcoholic Extracts on Trichomonas Gallinae[J].Veterinary Research Forum,2021,12(1):95-100.
[11] 熊天真,谢诗语,白蓉,等.薰衣草产酚酸内生真菌的分离及产物初步鉴定[J].食品与生物技术学报,2018,37(3):250-255.
[12] 曹宜,刘波,林营志,等.枯萎病尖孢镰刀菌的RAPD-PCR多态性分析[J].厦门大学学报,2004,8(43):74-78.
[13] 牛芳胜,马志强,毕秋艳,等.哈茨木霉菌与5种杀菌剂对番茄灰霉病菌的协同作用[J].农药学学报,2013,15(2):165-170.
[14] 宫占元,张国庆,于文莹,等. 哈茨木霉菌对水稻幼苗根际土壤微生物和酶活性的影响[J].干旱地区农业研究,2013,31(4):167-171.
[15] 马金慧,朱萍萍,茆振川,等.哈茨木霉菌株TRI2的鉴定及其对黄瓜根结线虫的防治作用[J].中国农学通报,2014,30(22):263-269.
[16] 关一鸣,潘晓曦,王莹,等.哈茨木霉菌、枯草芽孢杆菌对人参灰霉病和根腐病病原菌的拮抗作用[J].江苏农业科学,2014,42(5):123-125.
[17] 王雪,陈美兰,杨光,等.丛枝菌根真菌与哈茨木霉菌合用对连作丹参生长及质量的影响[J].中国中药杂志,2014,39(9):1574-1578.