Journal of Jishou University(Natural Sciences Edition)

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Key Genes for Polysaccharide Synthesis Pathway from Polygonatum Based on WGCNA

WANG Rong, MENG Ran, CHEN Xuemei, MENG Ying

Journal of Jishou University(Natural Sciences Edition) 2023, 44 (5): 64-71. DOI: 10.13438/j.cnki.jdzk.2023.05.009

Abstract （121）

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In order to find possible key genes related to the synthesis of polysaccharides in Polygonatum,this study explored the correlation between the expression patterns of core genes in leaves and rhizomes of Polygonatum cyrtonema Hua and Polygonatum odoratum (Mill.) Druce in combination with polysaccharide synthesis pathways from KEGG database using transcriptome data with expression difference and weighted gene co-expression network analysis (WGCNA).Our results indicated that FBA (fructose-bisphosphate aldolase) and GOLS (galactinol synthase) showed a strong correlation in Pearson linear regression calculation,which demonstrated that they are possible key genes for polysaccharide synthesis pathway in Polygonatum.

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Synthesis of Perylene Amide Alcohol Ester Under Microwave Irradiation

LIU Limin, SHI Jianjun, LIU Yang

Journal of Jishou University(Natural Sciences Edition) 2023, 44 (5): 72-76. DOI: 10.13438/j.cnki.jdzk.2023.05.010

Abstract （113）

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N-propyl-3,4,9,10-perylene terephthalic-3,4-alcohol ester-9,10-bisimide was synthesized under ultrasonic microwave radiation with N-propyl-3,4,9,10-perylene terephthalic acid and alkyl halide,aliphatic alcohol as solvent.Results showed that this reaction afforded corresponding compound with 65.7% yields.The optimized reaction condition was as follows:700 W of microwave power,60 min of reaction time,90℃of reaction temperature,and 80% of ultrasonic power.

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Isolation of Endophytic Bacteria from Inula Helenium L. and Identification of Its Antibacterial Activity

NAZIERBIEKE Wulumuhan, TUERDEBIEKE Mayila, BORRATHYBAY Entomack

Journal of Jishou University（Natural Sciences Edition) DOI: 10.13438/j.cnki.jdzk.2019.04.011

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Synthesis and Characterization of Sm(N-HPA)₃phen Complex and Its Fluorescence Properties

WANG Ying, MA Dongyun, GUO Yingxue, SONG Chunxiang，ZHANG Jie, LYU Yuguang

Journal of Jishou University（Natural Sciences Edition) DOI: 10.13438/j.cnki.jdzk.2019.04.012

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Performance Verification and Analysis of Electrophoretic Fluorimeter Based on AFP, AFP-L3 and PIVKA-Ⅱ

XING Yan, FENG Changhuan, DU Lijun

Journal of Jishou University（Natural Sciences Edition) DOI: 10.13438/j.cnki.jdzk.2019.04.013

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Distribution of the Newly Recorded Plant Polygonatum Sinopubescens in Hunan,China and Its Karyotype

XIANG Kuiwen,CHU Zhaofu,MENG Ran,YANG Huiling,ZHANG Daigui

Journal of Jishou University（Natural Sciences Edition) DOI: 10.13438/j.cnki.jdzk.2018.06.010

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Four Newly Recorded Species of Medicinal Plants in Hunan Province

YANG Huiling,MOU Cun,SHI Linjun,WANG Peng,ZHANG Daigui

Journal of Jishou University（Natural Sciences Edition) DOI: 10.13438/j.cnki.jdzk.2018.06.011

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Ultrasonic-Assisted Extraction of Protein from Trichosanthes Kirilowii Maxim

LIU Ailiang,DUAN Shaoxiong,XIONG Li'zhi

Journal of Jishou University（Natural Sciences Edition) DOI: 10.13438/j.cnki.jdzk.2018.06.012

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Effects of Short-Term Low Temperature on the Germination of Gynostemma Pentaphyllum Seeds

WU Xiaoyu，PENG Xiaolie，LIU Shibiao，LI Xiaoming，TIAN Xiangrong

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.04.007

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Ecological Risk Assessment of Heavy Metals in theSurface Sediments of Maozhou River

GONG Yalong,HUANG Cong,HUANG Lei,LI Hongyan,LIN Xiaoyan,XU Chuang,TAN Luming,ZHAO Liang

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.04.008

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Ecological Security Evaluation of Land in Nan County Based on Entropy

LEI Yanjin，SHUAI Hong

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.04.009

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First-Principle Study of the Electronic and Optical Properties of Ti2O3

YUAN Feng,ZHANG Haifeng,LU Shixiang

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.04.010

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Synthesis and Characterization of Titanium Incorporated Ordered Mesoporous Silicate Ti-KIT-6

WANG Bin,LI Lu,JIANG Chongwen,ZHONG Hong

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.04.011

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Recycling of Ammonia from Electrolytic Manganese Residue

LI Sisi，YANG Zhaoxia，LEI Yang，DAI Qinli

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.04.012

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Research Progress on the Electrode Material of Vanadium Redox Flow Batteries

ZHANG Wenze,WU Xianwen,WANG Yu’e,LUO Fei,CHENG Gang,HE Zhangxing

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.04.013

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Therapy Function of Aqueous Extract from Pteridium Aquilinum on Constipated Mice

LI Lihua，SHI Guang，DUAN Zhen，LV Jiangming，TIAN Xiangrong

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.03.012

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Composition Analysis and Quality Evaluation of Seed oil Extracted from Trichosanthes Kirilowii by Different Methods

LI Qiao，LI Gui，XIONG Lizhi，CHEN Gongxi

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.03.013

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Growth and Optical Properties of Tree Warty ZnO-Bi₂O₃ Complex Oxides Microcrystals

WU Zhifu,SHI Yunfeng,WANG Xueyang

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.03.014

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Synthesis，Characterization and Photochromism of Inorganic-Organic Hybrid Complexes

TENG Chunlin,ZANG Jianbin,YANG Zhijun,XUE Lian,LI Li,DENG Qian

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.03.015

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Screening and Identification of High Butanedione-Yielding Bacterial Strain

SU Guirong，ZHOU Lulu，YI Langbo，PENG Qingjing，PENG Qingzhong

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.02.010

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Experiment of Pressure Swing Adsorption of CO₂/CH₄at Low Temperature Through Coconut Shell Activated Carbon

LIU Xuewu,DU Baoguo,WU Jintao,WANG Xiaojuan,ZOU Jiupeng

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.02.011

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Electrochemical Reduction of Benzaldehyde to Phenylmethanol in Ionic Liquid BMIMBF₄

FENG Qiuju，LI Feng，XIAO Yonglai，LIU Renbo，CAO Ming，TIAN Jun

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.02.012

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Leaching Kinetics of Bornite in Acid Solution

XU Jiajian，LIU Zhixiong,DAI Dongyang,TANG Liandong，XIANG Yanhong,ZOU Xiaoyong

Journal of Jishou University（Natural Sciences Edition) DOI: 10.3969/j.cnki.jdxb.2016.02.013

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Numerical Simulation of the Spray Characteristics of Diesel,Methanol and Water Emulsion

WU Dong-Yin, WANG Wu-Qiang, LI Ming

journal6 2016, 37 (1): 60-63. DOI: 10.3969/j.cnki.jdxb.2016.01.014

Abstract （2090）

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This paper simulated the spray characteristics of diesel,methanol and water emulsion by Fluent,and obtained the particle trajectory figure and particle velocity vector diagram of the spray.Meanwhile,the influence of the spray injection pressure,environmental pressure and nozzle diameter is studied on the emulsion spray characteristics.This research provides certain guiding significance for the diesel,methanol and water emulsion application.

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Factors of Influencing Gemmae Germination and Growth of Huperzia Serrata

LUO Bing-Jie, YAO Mao-Jun, CAO De-Hua

journal6 2016, 37 (1): 64-68. DOI: 10.3969/j.cnki.jdxb.2016.01.015

Abstract （2070）

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The factors of influencing the gemmae germination of Huperzia serrata and growth condition are explored.The effect of culture medium,culture temperature,and light intensity and GA₃ on the gemmae germination condition of Huperzia was studied,and the growth morphogenesis during the course of gemmae germination of Huperzia serrata was observed at the same time.Results showed that the best culture temperature for the gemmae germination was (22±2)℃,and the germination rate of the optimum medium for Moore,the germination rate was as high as 82%,and the average plant length could be up to 14.620 mm.The second culture medium for the gemmae germination was Knudson C,the germination rate was 64%,and the average adventitious root length was about 5.158 mm.Darkness was conducive to gemmae germination,the germination rate was 92%,and the average adventitious root length was about 9.088 mm.Under 800lx weak light,the adventitious root grew slowly,and the adventitious root length could be up to 8.675 mm.1600lx light irradiation exhibited some inhibition for the adventitious root growth.And GA₃ for gemmae germination and growth exhibited some inhibition. This study establishes the gemmae germination culture system for Huperzia serrata,which consitutes the foundation for further study on the growth,physiological and biochemical change of Huperzia serrata.

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Characteristics Analyses of Kitchen Waste Leachate

WANG Ya-Guang, WU Yu-Xian, TENG Yao, HE Ze-Qiang, XIONG Li-Zhi

journal6 2016, 37 (1): 69-73. DOI: 10.3969/j.cnki.jdxb.2016.01.016

Abstract （3035）

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The kitchen wastes were collected from a student canteen in Jishou University to produce kitchen waste leachate in the laboratory in an anaerobic acidogenic reactor.The characteristics of kitchen waste leachate were analyzed.Results show that the concentration of COD and NH⁺₄—N for formed kitchen waste leachate after reaction for 8 weeks is more than 140.6 g/L and 16.2 g/L,respectively.With the increase of fermentation time,the pH of the kitchen waste leachate decreased from 5.9 in the end of 1^st week to 3.7 in the end of 8^th week.At the same time,the VFA concentration increased from 6.7 g/L to 17.9 g/L and the COD concentration increased from 57.9 g/L to 140.6 g/L,while the NH⁺₄—N concentration increased 0.3g/L to 16.2 g/L.Prolonging the reaction time,the pH of the kitchen waste leachate tends to be stable and the concentration of VFA and NH⁺₄—N increases slowly,while the concentration of COD has a downward trend.

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Content Determination and Ultrasonic Extraction Techniques of Total Flavonoids from Capsellae Bursa-Pastoris (L.)

LIU Ya-Qian, WEI Hua, LI Gui, HE Jian-Wu, CHEN Tao, ZHANG Yuan, ZHOU Fen

journal6 2016, 37 (1): 74-79. DOI: 10.3969/j.cnki.jdxb.2016.01.017

Abstract （1970）

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UV spectrophotometry for determination of total flavonoids was used.Via ultrasonic extraction technique,using NaNO3-Al(NO3) to detect the absorbance of samples and taking lutin as comparison,the contents of total flavonoids were determined.On the basis of single factor experiments,the orthogonal experiment was used in the optimization of technological parameters:extracting temperature,ethanol concentration,extracting time,the ratio of solvent to raw materials.The result showed that the contents of total flavonoids in different kinds of Capsella bursa-pastoris L. were from 0.49% to 3.83%.The contents of total flavonoids in flowers & fruits were the highest，and then in leaves and stems.The contents of total flavonoids in cultivated varireties were higher than those in wile varireties.The optimum extraction conditions were as follows：temperature at 65 ℃,50% ethanol concentration,ultrasonic extraction for 40 min,and the 25∶1 ratio of solvent to raw materials.In these conditions,the total flavonoids extraction rate was 89.26%.

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Combustion Method to Synthesize LiFePO₄ and Combustion Mechanism

CHEN Juan, SHI Yue, SUN Nian, CHEN Chang-Lian, WANG Shu-Lin, HUANG Zhi-Liang

journal6 2015, 36 (6): 53-58. DOI: 10.3969/j.cnki.jdxb.2015.06.013

Abstract （1587）

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Many traditional methods used to synthesize LiFePO₄ (LFP for short) have the disadvantages of long reaction time or unavoidable oxidation of Fe²⁺.In this work,the combustion method was developed to facilely synthesize LFP by using ferrous sulfate,diammonium hydrogen phosphate and lithium nitrate as main raw materials.The synthesized LFP was characterized by XRD and TG-DSC.The results showed that the combustion method could facilely synthesize purer LFP at 600 ℃ in a short period without the protection of inert gas.The studies on combustion mechanism indicated that from 120 ℃ to 290 ℃ the organic citric acid in the dry gel was combusted which could lead to a self-propagating combustion reaction and bring in carbon preventing the oxidation of Fe²⁺ simultaneously.And φ=n(CA)/n(LFP)=2 is the best amount of citric acid to synthesize LFP materials.

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Leaching Vanadium and Silicon from Stone Coal by Alkaline Process

ZENG Ying-Yuan, HUA Jun, YAN Wen-Bin, GAO Feng, CAI Jun

journal6 2015, 36 (6): 59-62. DOI: 10.3969/j.cnki.jdxb.2015.06.014

Abstract （1681）

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In order to make full use of valuable element silicon in stone coal,alkali leaching vanadium process was used in extracting vanadium and silicon from stone coal. Through pre-roasting,the stone coal structure can be destroyed and the leaching rate of vanadium and silicon can be improved.When the roast temperature was 850 ℃,the roast time was 2 h,the leaching temperature was 95 ℃,the leaching time was 4 h,the solid￣liquid ratio was 1∶1.4,and the ratio of alkali and ore was 1.2∶1,the leaching rates of vanadium and silicon were 86.6% and 61.4% respectively.

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Influential Factors on Nitrogen Migration and Transformation in Sedimen-Water Interface in the Dongjiang River

XIONG Lei, PENG Qing-Jing, YANG Li-Ping

journal6 2015, 36 (6): 62-68. DOI: 10.3969/j.cnki.jdxb.2015.06.015

Abstract （1795）

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This paper studies the nitrogen distribution in overlying water and sediment in the Dongguan segment of Dongjiang River.The indoor column experiment of nitrogen exchange between overlying water and sediment was conducted,and the influential factors on the nitrogen migration were investigated.The results indicated that TN concentration ranged from 2.13 to 4.48 mg/L,and nitrate was found to be the main form of contaminant,which accounted for 64.9% of TN.Sediment is an important source for ammonium and a sink for nitrate and nitrite.Illumination and disturbance are conducive to decrease the concentration of ammonia nitrogen;and higher temperature and initial pH value are conducive to the transformation of ammonia nitrogen.

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Mass Fraction of Chlorogenic Acid in the Leaves of Eucommia Ulmoides Oliver in Different Habitats of Xiangxi

LUO Hui-Guan, CHEN Yong-Wen, XIONG Li-Zhi

journal6 2015, 36 (6): 69-71. DOI: 10.3969/j.cnki.jdxb.2015.06.016

Abstract （1891）

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The contents of chlorogenic acid in leaves of Eucommia ulmoides Oliver from seven counties and one city in different habitats of Western Hunan were determined.The result showed that the contents of chlorogenic acid were significantly different in different habibats.The contents of chlorogenic acid were higher in the leaves of Eucommia ulmoides Oliver in the adrets of elevation 401~500 m，in light-grey soil，and with the parent material of limestone or sand shale.

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ESA/AMPS Copolymer Synthesis and Scale Inhibition Performance

LI Sen, YANG Jian-Jun, HE Ying-Xia, SONG Tian-Yu, XIANG Feng, WU Qing-Yun, ZHANG Jian-An, WU Ming-Yuan

journal6 2015, 36 (4): 44-47. DOI: 10.3969/j.cnki.jdxb.2015.04.012

Abstract （2222）

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ESA/AMPS copolymers has been synthesized with MA and AMPS as raw materials and ammonium persulfate as initiator.By static scale inhibition experiment，various experimental conditions on the inhibition rate of calcium carbonate scale and calcium phosphate scale by ESA/AMPS copolymers were examined.The results show that when monomer ratio m(MA)∶m(AMPS)=3∶1，the mass fraction of initiator is 4%，and the polymerization reacts for 3 h at 85~90 ℃,scale inhibition can achieve the optimum effect.

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Storability Differences Between Yellow and Black Seeds of Xiangzayou 631

LI Liang, MI Ze-Zhou, ZHANG Xiang, LI Xiao-Ming, TIAN Xiang-Rong

journal6 2015, 36 (4): 48-53. DOI: 10.3969/j.cnki.jdxb.2015.04.013

Abstract （2672）

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To investigate the relationship between seed color and storability，the artificial aged Xiangzayou 631 seeds have been used as materials to study the germination and antioxidative physiological and biochemical variation.Results showed that the germination rate of both yellow and black rape seeds decreased significantly after accelerated artificial aging，and germination of yellow seeds decreased dramatically faster than the black. Superoxide radicle production，contents of hydrogen peroxide and MDA increased by 3 d accelerated artificial aging treatment，activities of antioxidative enzyme (SOD and CAT) increased obviously，and the content of AsA declined obviously.The comparison between two color seeds showed superoxide radicle production，hydrogen peroxide contents of the yellow seeds were much higher than those of the black seeds，but the SOD activity and AsA content of yellow seeds were much lower than those of the black seeds.It can be concluded that the black seeds of Xiangzayou 631 possess stronger storability due to greater contents of nonenzymatic antioxidants.

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Classification and Identification of the Erysipelothrix Species Strains Based on the groEL Gene Sequences

YANG Zhenlong，NAZIERBIEKE Wulumuhan，BORRATHYBAY Enotmack

journal6 2015, 36 (4): 54-58. DOI: 10.3969/j.cnki.jdxb.2015.04.014

Abstract （2358）

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To investigate the effect of 16S rRNA gene and groEL gene used in the classification and identification of species of the genus Erysipelothrix，the 16S rRNA and groEL genes of four E.rhusiopathiae strains were amplified by PCR and sequenced and their phylogenetic threes was constructed.The open reading frame of groEL genes of four tested strains were 1 614 bp in length，and sequence similarities of the groEL gene between the four tested strains and the previously reported E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 were 99% and 86%，respectively.While the 16S rRNA genes of the four tested strains was 1 351 bp in length，and sequence similarity of the 16S rRNA gene between the four tested strains and E.rhusiopathiae type strain ATCC19414 and E.tonsillarum type strain ATCC43339 was 99%.Phylogenetic analysis indicated that the E.rhusiopathiae and E.tonsillarum were differentiated well by groEL gene sequence analysis.This study demonstrated that the groEL gene is an alternative phylogenetic marker for the classification of species of the genus Erysipelothrix.

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Recent Progress on Radical C—H Bond Cyclization to Form Oxindoles

ZHOU Dong, LI Yu-Hua, DENG You-Lin, LI Zhi-Hao- , TANG Shi

journal6 2015, 36 (2): 60-70. DOI: 10.3969/j.issn.1007-2985.2015.02.013

Abstract （2666）

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Oxindole and its derivatives are widely distributed in nature,and the synthetic method has been one of the hot topics of synthetic chemistry.According to the coupling mechanism,the researches on oxindole synthesis through free radical mediated C—H cyclization in recent years are summarized.In view of the effects of catalyst,oxidizing agent,and solvent on the reaction system,this paper discusses the method of cleaving C—H bond.

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Forest Resources,Its Culture and Application in Xiangxi Region

CHEN Gong-Xi, CHEN Xuan, ZHOU Qiang, XU Liang

journal6 2015, 36 (2): 71-76. DOI: 10.3969/j.issn.1007-2985.2015.02.014

Abstract （2308）

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Based on the actual investigation and literature review,it is found that in western Hunan,there are 101 families,321 genera and 101 species (including the cultivation of 8 families,29 genera and 76 species) of forest resources,which can be roughly divided into four categories of timber forests,gardening forests,economic forests,and firewood forests.Chinese fir occupies an important position in the western Hunan forest resources,with a total of about 10 kinds of 6 genera and 3 families being collectively referred to as “Chinese Fir”,which is not only known as “omnipotent woods”,but also has specific cultural meanings.Forest resources in Xiangxi region are significant in three aspects of the forestry economic,ecological maintenance and forest culture.

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Cloning and Sequencing of Adhesive Protein Genes of Avian Pasteurella Multocida

DUAN Shixiong,NAZIERBIEKE Wulumuhan,BORRATHYBAY Entomack

journal6 2015, 36 (2): 77-81. DOI: 10.3969/j.issn.1007-2985.2015.02.015

Abstract （2181）

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The genes encoding the outer membrane protein H (OmpH),lipoprotein E (PlpE),type 4 fimbrial subunit protein (PtfA) and fibronectin binding protein (PM1665) were amplified by PCR from genomic DNA of avian Pasteurella multocida P-1059,respectively.The PCR products were cloned into the pMD18-T vector and then sequenced.Sequence analyses showed that the open reading frames (ORFs) of the ompH,plpE,ptfA and pm1665 genes were 1 032,1 008,435 and 348 bp in length,and encoded the 343,335,144 and 115 amino acid residues.Nucleotide sequence homologies of the ompH,plpE,ptfA and pm1665 genes between the P-1059 and the previously reported different serotype strains of P. multocida were 95%~97%,95%~99%,94%~97% and 99%,respectively.In conclusion,the cloned genes encoding the adhesive proteins of avian P. multocida P-1059 will contribute to further study on pathogenesis of this organism.

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Effect of Selenium Enriched Fertilizer on Yields and Selenium Content of Peanut

LI Hui, TANG Jian-Zhou, YOU Yong, YANG Ji-Hui, XU Bin

Journal of Jishou University(Natural Sciences Edition) 2015, 36 (2): 82-85. DOI: 10.3969/j.issn.1007-2985.2015.02.016

Abstract （2524）

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The field experiment and instrument analysis method to study the trace element selenium compound fertilizer influence and the yield and selenium content of peanut in Hepu of Beihai.The results showed that:in the flowering period and flowering period of peanuts,sprayed the Selenium enriched fertilizer,could significantly increase the content of peanut selenium.Selenium content increased with the frequency of spraying,the selenium content is 1.031 μg/g,improved more than times of conventional control.In the meanwhile,the yields was increased six percent.It can make the selenium content in selenium enriched standard to peanut by sprayed Selenium enriched fertilizer.

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Niltava Sundara：A New Bird Record in Hunan Province

WANG Ben-Zhong, PENG Xing-Wen, PENG Le, LIU Hui, ZHANG You-Xiang

journal6 2015, 36 (2): 86-87. DOI: 10.3969/j.issn.1007-2985.2015.02.017

Abstract （2853）

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Niltava sundara,a Passerine bird taken by photography during the field investigation of avian diversity in the Xiaoxi National Nature Reserve of Hunan Province（110°21′91″E，28°78′01″N，380 m asl）on Februry 19^th of 2014,is the first record of Hunan avifauna.

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Different Expressions of vscO Gene from Vibrio Alginolyticus in Different Environments

PANG Huan-Ying, ZHOU Ze-Jun, DING Yu, HUANG Yu-Cong, WU Zao-He, JIAN Ji-Chang

journal6 2014, 35 (4): 59-63. DOI: 10.3969/j.issn.1007-2985.2014.04.014

Abstract （4262）

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To understand the impact of environmental factors on the expressions of vscO gene,the mRNA expressions of vscO gene in different growth periods and with environmental factors,such as temperature,salinity and pH,were detected by RT-PCR method. The results indicated that the mRNA expression of vscO reached a maximum at 28 ℃ in salinity of 2%,with the initial medium pH 7.0 when V. alginolyticus was in the middle phase of growth.

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