Journal of Jishou University(Natural Sciences Edition)

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Screening and Identification of High Butanedione-Yielding Bacterial Strain

SU Guirong,ZHOU Lulu,YI Langbo,PENG Qingjing,PENG Qingzhong   

  1. (College of Biology and Environmental Science,Jishou University,Jishou 416000,Hunan China)
  • Online:2016-03-25 Published:2016-05-11


To obtain the high butanedione-yielding bacterial strain,41 strains of bacteria isolated from traditional Tujia bacon were screened by way of V-P color reaction and 1,2-diaminobenzene colorimetric method.The isolated strains were identified by bacterial morphological,physiological and biochemical characteristics,and phylogenetic relationships based on 16S rRNA gene sequences.It was found that 7 strains of bacteria could produce higher content of butanedione,of which strain BCL-8 had the highest yield of butanedione.When strain BCL-8 had been fermented in glucose and peptone medium for 20 hours,butanedione content in fermentation liquor was determined to be 68.9 mg/L.Strain BCL-8 was identified as Bacillus licheniformis based on its morphological,physiological and biochemical characteristics,and 16S rRNA gene sequences analysis.The results showed that strain BCL-8 is a superior butanedione-producing microorganism.

Key words: butanedione, Bacillus licheniformis, screening, identification

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