journal6 ›› 2014, Vol. 35 ›› Issue (5): 51-54.DOI: 10.3969/j.issn.1007-2985.2014.05.012

• Biological resources • Previous Articles     Next Articles

Screening and Phylogenetic Analysis of Amylase-Producing Strains Isolated from the Air Samples from a Liquor Starter-Making Workshop of Jiugui Liquor Co. Ltd.

 ZHONG  Xiao-Juan, YU  Bing, LIU  He, XIAO  Jian-Qing, LI  Hong-Jun, FAN  Lin, CHENG  Jin-Lian, LIU  Zhu-Xiang, CHEN  Yi-Guang   

  1.  (1.College of Biology and Environmental Sciences,Jishou University,Jishou 416000,China;2.Jiugui Liquor Co. Ltd.,Jishou 416000,China)
  • Online:2014-09-25 Published:2014-10-30

Abstract: The culturable bacteria in the air samples from a liquor starter-making workshop in Jiugui Liquor Co.Ltd.,China were isolated by using the natural precipitation method and culture-dependent method.The amylase-producing ability of the strains was investigated by using the plate hydrolysis spot method.The results showed that 16 out of 65 (24.6%) strains tested exhibited amylase-producing activity,in which 4 strains (JSM 2145008,JSM 2155001,JSM 2155017,JSM 2175012) could relatively strongly produce amylase.Subsequently,these 4 strains were investigated by means of a phylogenetic analysis based on 16S rRNA gene sequence comparisons.The results showed that these 4 strains were the members of 3 families (Bacillaceae,Deinococcaceae,Micrococcaceae) in 3 phylogenetic group/phyla (Actinobacteria,Deinococcus-Thermus,Firmicutes):strain JSM 2145008 belonged to the genus Arthrobacter (Actinobacteria,Micrococcaceae),being most closely to the type strain of Arthrobacter oxydans (sequence similarity 99.8%);strains JSM 2155001 and JSM 2175012 were the members of the genus Deinococcus (Deinococcus-Thermus,Deinococcaceae),being related most closely to the type strains of Deinococcus daejeonensis (99.6%) and Deinococcus yunweiensis (99.9%),respectively;strain JSM 2155017 belonged to the genus Bacillus (Firmicutes,Bacillaceae),exhibiting 100% 16S rRNA gene sequence similarity to the type strain of Bacillus stratosphericu.In conclusion,there is abundant bacterial diversity,as well as a number of amylase-producing strains in the air samples from a liquor starter-making workshop in Jiugui Liquor Co. Ltd.

Key words: liquor starter-making workshop, airborne microbes, amylase activity, phylogenetic analysis

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