journal6 ›› 2014, Vol. 35 ›› Issue (6): 73-77.DOI: 10.3969/j.issn.1007-2985.2014.06.018

• Biological resources • Previous Articles     Next Articles

Screening and Phylogenetic Analysis of Antibiotic-Producing Halophilic and Halotolerant Bacteria from the Dehang Canyon in Hunan China

LI  Hong-Jun, XIAO  Jian-Qing, CHENG  Jin-Lian, LIU  He, ZHONG  Xiao-Juan, YI  Min, HE  Jian-Wu, LIU  Zhu-Xiang, CHEN  Yi-Guang   

  1.  (1.College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China;2.Jiugui Liquor Co.Ltd.,Jishou 416000,Huana China)
  • Online:2014-11-25 Published:2014-11-27

Abstract: The antimicrobial activity of the fermentation brothes of 294 halophilic and halotolerant bacteria,isolated from ordinary non-saline soils collected from the Dehang Canyon (28°15′~28°43′ N,109°30′~109°45′ E) in Hunan Province,China,was screened using the agar diffusion method employing 8 indicator microorganisms (three Gram-positive bacteria,three Gram-negative bacteria and two fungi).The results showed that,out of 294 strains tested,109 were positive in antimicrobial activity (37.1%),in which 5 strains exhibited strong antimicrobial activity.Then these 5 strains were submitted for 16S rRNA gene amplification and phylogenetic analysis after phenotypic characterization including morphological,biochemical and physiological tests.The results suggested that these 5 strains were members of 4 genera in 3 families (Bacillaceae,Halomonadaceae,Nocardiopsaceae) of 3 phyla (Actinobacteria,Firmicutes,Proteobacteria):strain JSM 102030 belonged to the genus Halobacillus (Firmicutes,Bacillaceae),being most closely to the type strain of Halobacillus trueperi (sequence similarity 99.1%);strains 102052 and JSM 102054 were members of the genus Halomonas (Proteobacteria,Halomonadaceae),being related most closely to the type strain of Halomonas alkaliphila (98.3%);strains JSM 102066 strain JSM 2155017 belonged to the genus Nocardiopsis (Actinobacteria,Nocardiopsaceae),exhibiting 99.3 % 16S rRNA gene sequence similarity to Nocardiopsis prasina DSM43845T;strain JSM 102082 belonged to the genus Oceanobacillus (Firmicutes,Bacillaceae),exhibiting 99.6% 16S rRNA gene sequence similarity to Oceanobacillus kimchii X50T.

Key words: ordinary non-saline environment, halophilic bacteria, halotolerant bacteria, antimicrobial activity, phylogenetic analysis

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