Isolation and Plant Growth-Promoting Ability of ACC Deaminase-Containing Bacteria from Rhizosphere of  Gynostemma Pentaphyllum Makino

doi:10.3969/j.issn.1007-2985.2014.05.011

Abstract

Abstract: A total of 9 bacterial strains were isolated from rhizosphere of Gynostemma pentaphyllum through enrichment on 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source.ACC deaminase assay showed that all isolates differed in their potential for ACC deaminse activity,among which 3 strains were positive for siderosphere by chrome azurol S (CAS) assay.Inoculation of rice seedlings with 7 ACC deaminase-containing strains had significant effect on root elongation and root fresh weight,among which the strain JDG127 caused 1.6-fold increase in root length and root fresh weight over uninoculated control.Based on the physiological and biochemical characteristics in combination with 16S rRNA gene sequences analysis,the 9 ACC deaminase- containing strains were identified as Stenotrophomonas (1 strain),Paenibacillus (1 strain),Bacillus (1 strain),Enterobacter (2 strains) and Acinetobacter (4 strains).The results indicated that the siderosphere producing ability of rhizobacteria may be correlated with their plant growth-promoting ability when compared to their ACC-deaminase activity.

Key words: Gynostemma pentaphyllum, rhizobacteria, ACC deaminase, growth-promoting ability

SONG Jin-Qiu, WU Lu-Mu-Han-·Na-Zi-er-Bie-Ke, ZHANG Ti, En-Te-Ma-Ke-·Bu-La-Ti-Bai . Isolation and Plant Growth-Promoting Ability of ACC Deaminase-Containing Bacteria from Rhizosphere of Gynostemma Pentaphyllum Makino[J]. journal6, 2014, 35(5): 43-50.

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Isolation and Plant Growth-Promoting Ability of ACC Deaminase-Containing Bacteria from Rhizosphere of Gynostemma Pentaphyllum Makino

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