Isolation of Endophytic Bacteria from Inula Helenium L. and Identification of Its Antibacterial Activity

doi:10.13438/j.cnki.jdzk.2019.04.011

Abstract

Abstract:

Using the tissue separation method, 32 endophytic bacteria were isolated from the roots, stems and leaves of Inula helenium L., and antibacterial activiy of these strains were determined by using the agar disc diffusion test. Identification of these strains was determined by their morphological, physiological-biochemical properties and phylogenetic analysis based on 16S rRNA sequences. The results showed that the 10 endophytic bacteria had remarkable antibacterial activities to Escherichia coli, Bacillus subtilis and Pasteurella multocida. The endophytic strains YIR-5, YIR-7, YIR-8 and YIR-9 were classified as genus Pseudomonas, strains YIR-3 and YIR-6 as Serratia plymuthica, strains YIR-4, YIS-1 and YIS-2 as Rahnella aquatilis, Bacillus halotolerans and Bacillus cereus respectively, and the strain YIL-3 as the genus Bacillus. There are a variety of endophytic bacteria from Inula helenium L., and Bacillus mojavensis YIS-1 has the greatest antibacterial activity for escherichia coli, bacillus subtilis, and avian pasteurella multocida.

Key words: Inula helenium L., endophytic bacteria, antibacterial activity, identification

NAZIERBIEKE Wulumuhan, TUERDEBIEKE Mayila, BORRATHYBAY Entomack. Isolation of Endophytic Bacteria from Inula Helenium L. and Identification of Its Antibacterial Activity[J]. Journal of Jishou University（Natural Sciences Edition), DOI: 10.13438/j.cnki.jdzk.2019.04.011.

References

[1] STURZ A V, NOWAK J. Endophytic Communities of Rhizobacteria and the Strategies Required to Create Yield Enhancing Associations with Corps[J]. Applied Soil Ecology, 2000, 15(2): 183-190.
[2] 王莉衡.植物内生菌及其次生代谢活性物质多样性的研究进展[J].化学与生物工程，2012,29(10):1-3.
[3] BALLIO A, BOSSA F, DIGIOGIO P, et al. Sructure of the Pseudomycins， New Lipodepsipeptides Produced by Pseudomonas Syringae MSU16H[J]. FEBS Letter, 1994, 355: 96-100.
[4] MILLER C M, MILLER R V, GARTON-KENNY D, et al.Ecomycins,Unique Antimycotics from Pseudomonas Viridiflava[J]. Journal of Applied Microbiology, 1998, 84(6): 937-944.
[5] 国家药典委员会.中华人民共和国药典：一部[M].北京:中国医药科技出版社,2015:253.
[6] HUO Y, SHI H, LI W, et al. HPLC Determination and NMR Structural Elucidation of Sesquiterpene Lactones in Lnula Helenium[J]. Journal of Pharmaceutical and Biomedical Analysis, 2010, 51(4): 942-946.
[7] 张嫚丽,霍长虹,刘丽,等.土木香药材HPLC指纹图谱研究及土木香内脂和异土木香内酯测定[J].中草药,2010,41(9):15-39.
[8] DORN D C, ALEXENIZER M, HENGSTLER J G, et al. Tumor Cell Specific Toxicity of Lnula Helenium Extracts[J]. Phytotherapy Research, 2006, 20(11): 970-980.
[9] LI Y, NI ZY, ZHU MC, et al. Antitumour Activities of Sesquiterpene Lactones from Lnula Helenium and Lnula Japonica[J]. Zeitschrift Für Naturforschung C, 2012, 67(7/8): 375-380.
[10] HUO Y, SHI H M, WANG M Y, et al. Chemical Constituents and Pharmacological Properties of Radix Inulae[J]. Die Pharmazie, 2008, 63(10): 699-703.
[11] RHOADES K R, RIMLER R B. Capsular Groups of Pasteurella Multocida Isolated from Avian Hosts[J]. Avian Diseases, 1987, 31(4): 895-898.
[12] MYINT A, CARTER G R, JONES T O. Prevention of Experimental Haemorrhagic Septicaemia with a Live Vaccine[J]. The Veterinary Record, 1987, 120(2): 500-501.
[13] AVAKIAN A P, DICK J W, DERIEUX W T. Fowl Cholera Immunity Induced by Various Vaccines in Broiler Minibreeder Chickens Determined by Enzyme-Linked Immunosorbent Assay[J]. Avian Diseases, 1994, 33(1): 97-102.
[14] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.

[1]	HE Renwang, ZHU Xiaojun, SUN Jie, LU Yingjian, QIN Junlong. Intelligent Weighing and Pricing System Design with User Recognition Based on RFID [J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(5): 42-47.
[2]	WANG Bo, DING Fang, LIU Mingyan, TIAN Miaofa. Optimal Slip Ratio Identification Estimator Design Based on μ-S Model [J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(5): 48-56.
[3]	CHEN Tianyi, BAIDOULA·Nulan, TUERDEBIEKE·Mayila, ENTOMACKY·Borrathyba. Antibacterial Activity and Identification of Endophytic Fungi YDZ-8 Isolated from Lavandula angustifolia Mill. [J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(2): 47-51.
[4]	YANG Dequan, SHE Yonghong, JI Linfang, DING Ye. Thin Layer Chromatographic Identification of Cynanchum otophyllum Decoction Pieces and the Standard Limit of Extraction Inspection [J]. Journal of Jishou University(Natural Sciences Edition), 2022, 43(5): 43-46.
[5]	HAN Xiangyun, YANG Dequan, QIN Aitao, XU Yuhui. Simultaneous TLC Identification of Paulownia Leaf and Sesame Oil in Compound Tongye Burn Oil [J]. Journal of Jishou University(Natural Sciences Edition), 2022, 43(5): 52-55.
[6]	LIAO Guangkai, ZHANG Zheng, NIU Yibo, SONG Zhiguo. Vehicle Re-Identification Based on Multi-Scale and Wavelet Spatial Attention [J]. Journal of Jishou University(Natural Sciences Edition), 2021, 42(6): 15-22.
[7]	SU Xiangfu. Empirical Study on Factors Influencing Identification of Ideological and Political Education in Colleges and Universities Based on 5W Model [J]. Journal of Jishou University（Natural Sciences Edition), 2019, 40(6): 90-96.
[8]	SONG Haorui, LI Yan, WANG Le, YAO Yifan. Optimum Cultivation Conditions for Extracellular Polysaccharide in Lactobacillus [J]. Journal of Jishou University（Natural Sciences Edition), 2019, 40(5): 59-62.
[9]	MIAO Fengjuan,LU Xiaoxu,TAO Bairui. Design of Rice Traceability System Based on WSN and RFID [J]. Journal of Jishou University（Natural Sciences Edition), 2019, 40(3): 33-38.
[10]	DONG Hui,MA Jian,FANG Xiao,YAO Hongliang. RFID Data Stream Cleaning Algorithm of Internet of Ships Based on MBF [J]. Journal of Jishou University（Natural Sciences Edition), 2018, 39(4): 20-24.
[11]	PENG Qiaomu,ZHOU Lulu,XIONG Li,PENG Qingzhong,HU Guang. Screening of a Methylotrophic Bacteria Serratia Marcescens NH8 and Isolation of Its Pyrroloquinoline Quinone (pqq) Genes Cluster [J]. Journal of Jishou University（Natural Sciences Edition), 2018, 39(1): 59-64.
[12]	REYINBAIKE Asaimuguli,NAZIERBIEKE Wulumuhan,BORRATHYBAY Entomack. Antimicrobial Activity and Identification of Endophytic Fungi Strain YIGZ-3 Isolated from Inula Helenium L. [J]. Journal of Jishou University（Natural Sciences Edition), 2017, 38(5): 71-76.
[13]	XIONG Wuguo,LI Taibing,LI Jiaxing,DENG Chong,HUANG Cheng. Extraction Conditions and Determination of Antibacterial Activity in Vitro of Konjac Glucomannan [J]. Journal of Jishou University（Natural Sciences Edition), 2017, 38(3): 60-63.
[14]	HE Qian，ZHENG Jie，TIAN Yungang，LIU Yihan，WANG Jianxia，WEI Hua . Separation and Preparation of a C₁₅ Triolefinic Urushiol from Raw Lacquer [J]. Journal of Jishou University（Natural Sciences Edition), 2016, 37(5): 42-44.
[15]	SU Guirong，ZHOU Lulu，YI Langbo，PENG Qingjing，PENG Qingzhong. Screening and Identification of High Butanedione-Yielding Bacterial Strain [J]. Journal of Jishou University（Natural Sciences Edition), 2016, 37(2): 38-42.