Methods in the Preparation of F-Type ATP Synthase

Abstract

Abstract: As the energy currency of the cell,ATP is mainly produced by the catalysis of ATP synthase in the mitochondria.In the past 50 years,scientists have done much on ATP synthase’s structure and its function already,but people have known more about function but fewer about the mechanism,so there is much more work to do.High purified ATPase which should come first is the key factor in the study of enzyme’s structure and catalytic mechanism.So isolation of the ATP synthase has become the primary work for the researchers who want to study it.This article has summarized and introduced some valuable methods in the isolation of mitochondria and submitochondria,and purification of ATP synthase,and the assay of the enzyme’s activities in details respectively.

Key words: mitochondria, submitochondrial particles, ATP synthase, extraction, purification, activity

ZHU Jie- . Methods in the Preparation of F-Type ATP Synthase[J]. journal6, 2005, 26(2): 24-28.

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