Abstract: Chlorogenic acid and flavonoids were simultaneously extracted by ethanol with the aid of ultrasonic treatment from leaves and rattan of Lonicera japonica and then isolated by ethyl ecetate and macroporous resin D101.The purification of soluble flavonoid and chlorogenic acid was achieved by re-crystallization with ethanol and N-Hexane phase separation,respectively.Results showed that two products (A and B) were obtained and identified to be chlorogenic acid and soluble flavonoid.The chlorogenic acid content in product A was 91.2% with a yield of 1.23% and the flavonoid content in product B was 84.5% with a yield of 0.50%.The proposed method has been used to obtain flavonoids and chlorogenic acid from leaves and rattan of Lonicera japonica with satisfactory results.

Key words: leaves and rattan of Lonicera japonica, flavonoid, chlorogenic acid, macroporous resin