journal6 ›› 2015, Vol. 36 ›› Issue (3): 82-89.DOI: 10.3969/j.issn.1007-2985.2015.03.018

• Biological resources • Previous Articles     Next Articles

Separation and Characterization of Catechin in man-Made Green Tea,the Fruits and Leaves of Berchemia Floribunda with Macroporous Resin and HPLC

 YAO  Shu-Feng, TANG  Ke-Hua, LIU  Xiao-Pan, CHENG  Jiang, DONG  Ai-Wen   

  1. (Key Laboratory of Forest Products and Chemical Engineering of Hunan Province,Jishou University,Zhangjiajie 427000,Hunan China)
  • Online:2015-05-25 Published:2015-06-03

Abstract: In this study,the extraction process of catechins was investigated with a certain concentration of ethanol as solvent from man-made green tea,fruits and leaves of Berchemia floribunda,using the method of waterbath.After the fat soluble pigment was removed by petroleum ether from extracting solution,the catechins was purified by porous resin adsorption method.The static adsorption and desorption tests of catechin with macroporous resin showed that D941 was the optimal resin for purification and the pure water was the optimum eluant.Purified liquid of fruit,leaves and home-made green tea extracts were detected by HPLC.The catechins was separated on PC18 chromatographic column (4.6×250 mm,5 μm) at 35 ℃,with nitrile-0.4% phosphoric acid (13∶87) as mobile phase at a flow rate of 1 mL/min,and detected at the wavelength of 279 nm (UV scan).The results showed that the catechin content of fruit was the lowest,while the content of man-made green tea was significantly higher than that of both the fruit and leaves through the optimized extraction processes.

Key words: Berchemia floribunda, catechin, macroporous resin, HPLC

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