Separation and Characterization of Catechin in man-Made Green Tea,the Fruits and Leaves of Berchemia Floribunda with Macroporous Resin and HPLC

doi:10.3969/j.issn.1007-2985.2015.03.018

Abstract

Abstract: In this study,the extraction process of catechins was investigated with a certain concentration of ethanol as solvent from man-made green tea，fruits and leaves of Berchemia floribunda,using the method of waterbath.After the fat soluble pigment was removed by petroleum ether from extracting solution,the catechins was purified by porous resin adsorption method.The static adsorption and desorption tests of catechin with macroporous resin showed that D941 was the optimal resin for purification and the pure water was the optimum eluant.Purified liquid of fruit,leaves and home-made green tea extracts were detected by HPLC.The catechins was separated on PC18 chromatographic column (4.6×250 mm,5 μm) at 35 ℃,with nitrile-0.4% phosphoric acid (13∶87) as mobile phase at a flow rate of 1 mL/min,and detected at the wavelength of 279 nm (UV scan).The results showed that the catechin content of fruit was the lowest,while the content of man-made green tea was significantly higher than that of both the fruit and leaves through the optimized extraction processes.

Key words: Berchemia floribunda, catechin, macroporous resin, HPLC

YAO Shu-Feng, TANG Ke-Hua, LIU Xiao-Pan, CHENG Jiang, DONG Ai-Wen. Separation and Characterization of Catechin in man-Made Green Tea,the Fruits and Leaves of Berchemia Floribunda with Macroporous Resin and HPLC[J]. journal6, 2015, 36(3): 82-89.

References

[1] 陈立，董俊兴.勾儿茶属植物化学成分及其生物活性研究进展[J].中草药，2006,37(4):627-630.

[2] 原征彦.茶多酚类的功能和食品中应用[J].新食品工业，1990，32(2)：33-38.

[3] 杨贤强，曹明富，沈生荣，等.茶多酚生物活性的研究[J].茶叶科学，1993，13(1)：51-59.

[4] 王杰.普洱茶提取茶多酚及其脱咖啡碱的研究[D].广州：华南理工大学,2012.

[5] 朱斌，陈晓光，宋航，等.茶多酚制备高纯度EGCG的工艺研究[J].食品研究与开发，2009，30(4)：7-9.

[6] 王慧，蒋莲芬，唐超，等.高山绿茶茶多酚提取工艺研究[J].安徽农业科学,2012，40(7)：4 007-4 009.

[7] 陈宗懋.茶多酚类化合物抗癌的生物化学和分子生物学基础[J].茶叶科学，2003，23(2)：83-93.

[8] 刘丽霞.茶叶中6种主要儿茶素的高效液相色谱方法建立及应用[D].南京：南京理工大学，2013.

[9] DONG Aiwen，ZHOU Qi，BU Xiaoying,et al.Adsorption Properties for Separation of Apigenin from Viola Yedoensis on LSA-10 Resin[J].Chinese Herbal Medicines，2014，6(1)：60-66.

[10] 干宁，李榕生，陈亚东,等.分子印迹固相萃取和电喷雾质谱法联用测定茶叶中四种儿茶素[J].茶叶科学，2009，29（3）：231-235.

[11] 沈晓玲,龚正礼.大孔树脂在茶叶功能成分分离中的应用进展[J].食品工业科技，2013，34（16）：396-399.

[12] 张盛，刘仲华，黄建安，等.吸附树脂法制备高纯儿茶素的研究[J].茶叶科学,2002，22(2)：125- 130.

[13] 徐仲溪，刘仲华，王坤波，等.绿茶多酚和儿茶素提取与原料品质关系的研究[J].湖南农业大学学报：自然科学版，2004，30（3）：257-260.

[14] 贾智若，李兵，李耀华，等.反相高效液相色谱法测定杜仲中儿茶素的含量[J].中国实验方剂学杂志，2013，19(5)：117-119.

[1]	Deyu Xie, Seyit Yuzuak, Yue Zhu, Qingzhong Peng. Anti-COVID-19 Pandemic Effect of Plant Flavonoids: Use of Green Tea Flavonoids [J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(2): 64-86.
[2]	QIN Aitao, YANG Dequan, HAN Xiangyun, XU Yuhui. Determination of Sesamin in Compound Tongye Burn Oil by HPLC [J]. Journal of Jishou University(Natural Sciences Edition), 2022, 43(5): 47-51.
[3]	XU Yuhui, ZHOU Jiani, QIN Aitao, HAN Xiangyun, YANG Dequan. HPLC Determination of Ursolic Acid and Oleanolic Acid in Paulownia Leaves from Xiangxi [J]. Journal of Jishou University(Natural Sciences Edition), 2022, 43(4): 92-96.
[4]	ZHANG Xiaoxu，OUYANG Yuzhu，NIE Pengcheng，TANG Ningmei. Separation Technology of Oleanolic Acid in Akebia Trifoliata Peel by Macroporous Resin [J]. Journal of Jishou University（Natural Sciences Edition), 2016, 37(6): 56-61.
[5]	LUO You, CHEN Li-Hua, LIANG Xuan, HUANG Hao, LI Biao, HUANG Lian. Simultaneous Extraction and Isolation of Chlorogenic Acid and Flavonoids from Leaves and Rattan of Lonicera Japonica [J]. journal6, 2011, 32(5): 91-94.
[6]	LI Xiang-Li, XIAO Kai-Jun, HU Xian-Yun, LUO Xin-Yi, GUO Si-Yuan. Metabolic Dynamic of Camptothecin and 10-Hydroxycamptothecin in Camptotheca acuminata Decne Leaves During the Process of Growth [J]. journal6, 2009, 30(5): 87-92.
[7]	LI Ya-Nan, LI Hui, SHI Hui, ZHANG Chao-Hui, HU Wen-Bin, LI Zhi-Ping. Analogue Recognition and Chromatographic Behaviors of MolecularlyImprinted Polymer for Protocatechuic Acid [J]. journal6, 2008, 29(4): 75-82.
[8]	LI Wei, CAO Yong, WEI Hua, ZHOU Dong-Wu. Reversed-Phase HPLC Determination of Shikimic Acid in Illicium Verum Hook.F. [J]. journal6, 2008, 29(1): 104-106.
[9]	QIU Yan-Lin, HU Jing, MIAO Jin-Feng, LIANG Liang. Separation of Glutathione from Abandoned Beer Yeast by Macroporous Resin [J]. journal6, 2008, 29(1): 83-86.
[10]	LI Hui, LI Ya-Nan, LONG Ling-Liang, YAO Zu-Feng, OUYang Yu-Zhu , LI Zhi-Ping. Solvent Effect on Extraction Efficiency of Chlorogenic Acid from the Leaves of Eucommia Ulmodies by Ultrasonically Assisted Extraction Technique [J]. journal6, 2006, 27(4): 90-92.
[11]	PENG Yong-Mei, HE Yong-Mei. Determination of Puerarin in Xingmaitong Drop Pills by HPLC [J]. journal6, 2006, 27(3): 117-118.
[12]	LIU Jun, ZHANG Jie, CAO Mei, LI Da-Xu, YANG Zhi-Rong, ZHAO Jian. Study on the Separation and Distribution ofα-amanitin in Amanita virosa [J]. journal6, 2004, 25(4): 39-43.
[13]	JIANG Jian-Bo. Determination of Cefradin by RP-HPLC [J]. journal6, 2003, 24(3): 94-96.
[14]	PENG Xiao-Chun, PENG Qing-Jing, LI Hong-Xiong. Determination of Flavonol Glycosides of Ginkgo Biloba Explants [J]. journal6, 2003, 24(1): 60-62.
[15]	PENG Xiao-Chun, WANG Hui-Xian, CHEN Shi-Guo, ZHOU Sai-Qin. Studies on the Efficacious Chemical Constituents of Medicinal of S. davidii [J]. journal6, 2001, 22(3): 59-60.