Abstract: Endophytic fungi of the cultured tissue from Huperzia serrata were isolated and identified by observing the appearance shape of colonies according to the Identification Handbook of Fungus.In this study,the endophytic fungi were cultured in PDA medium and purified by the methods of agar plate.At the same time the shape of these fungi was observed and identified by microphotograph technology.The results showed that there were seven endophytic fungi species isolated from the cultured tissue of the tip stem of Huperzia serrata,which respectively belonged to Peziza Dill.ex Fr,Paecilomyces Bainier,Saccardia Cooke,A.ustus,P.chrysogenim,Coniothecium Cda.

Key words: huperzia serratta(thunb)trev., endophytic fungi, isolation, identification