Abstract: Macroporous resin D001 was adopted for separation of GSH from abandoned beer yeast.The result indicated that optimum technological parameters of separation was that pH of 0.02 mol/L phosphate buffer was 4.5 for adsorption ,while 7.5 for elution,and the elution ratio was 2.9 mL/min.The raw product was white,the purity of the GSH was 28.47% analysised by HPLC,and the reclaimed ratio was 71.6%.

Key words: glutathione, macroporous resin, abandoned beer yeast