Screening and Identification of High Butanedione-Yielding Bacterial Strain

doi:10.3969/j.cnki.jdxb.2016.02.010

Abstract

Abstract:

To obtain the high butanedione-yielding bacterial strain,41 strains of bacteria isolated from traditional Tujia bacon were screened by way of V-P color reaction and 1，2-diaminobenzene colorimetric method.The isolated strains were identified by bacterial morphological,physiological and biochemical characteristics，and phylogenetic relationships based on 16S rRNA gene sequences.It was found that 7 strains of bacteria could produce higher content of butanedione，of which strain BCL-8 had the highest yield of butanedione.When strain BCL-8 had been fermented in glucose and peptone medium for 20 hours，butanedione content in fermentation liquor was determined to be 68.9 mg/L.Strain BCL-8 was identified as Bacillus licheniformis based on its morphological，physiological and biochemical characteristics，and 16S rRNA gene sequences analysis.The results showed that strain BCL-8 is a superior butanedione-producing microorganism.

Key words: butanedione, Bacillus licheniformis, screening, identification

SU Guirong，ZHOU Lulu，YI Langbo，PENG Qingjing，PENG Qingzhong. Screening and Identification of High Butanedione-Yielding Bacterial Strain[J]. Journal of Jishou University（Natural Sciences Edition), DOI: 10.3969/j.cnki.jdxb.2016.02.010.

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