Abstract: Method of separat ion of a hydroxyphenyl- 21H, 23H- porphine[ 5- ( 4- hydroxyphenyl) - 10, 15, 20-triphenyl- 21H, 23H- porphine( H₂TPPOH) ] and many hydroxyphenyl- 21H, 23H- porphine[ 5, 10- di( 4- hydroxyphenyl) - 15, 20- diphenyl- 21H, 23H- porphine( H₂TPP( OH) 2) ; 5- phenyl- 10, 15, 20- tri( 4- hydrox yphenyl) - 21H, 23H- porphine( H₂TPP( OH) ₃) ] was improved.The results showed that in the course of separating all kinds of hydroxyphenyl porphine, by the new method, the separate efficiency and product purity were better, the using amount of eluant was less and the column separation times was shorter. In addition, the influence of the ratio of reactants, drop speed of pyrrole solution and operational method on the yield of synthesing all kinds of hydroxyphenyl porphine were inves  tigated,wi th 3:1. 05:4( C₆H₄CHO: HOC₆H₄CHO:C₄H₅N, mole ratio) , drop pyrrole solution in 3 minutes and strong motor stir, the yield of the H₂TPPOH was increased.

Key words: 4- hydroxyphenyl-21H, 23H-porphine, synthesis, separation