Abstract: This experiment was conducted to study MDA content and the producing rate of superoxide anion free radical (·O₂) in the leaves of the Epimedium sagittatum M. from Feng Huang、Yong Shun and Long Shan in wester Hunan of China.The results showed that the producing rate of ·O2 from Yong Shun was the highest (0.057 1±0.003　3 μmol·min^-1·g^-1),and the producing rate of ·O2 from Long Shan was the lowest (0.053　0±0.000　9 μmol·min^-1·g^-1);but MDA content from Long Shan was the highest (0.014　9±0.001　4 nmol·g^-1),and MDA content from Feng Huang was the lowest (0.007　5±0.001　4 nmol·g^-1)
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Key words: superoxide anion free radical, MDA, different populations, epimedium sagittatum M.