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For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Research on Gastrointestinal Toxicity Evaluation of Dichroa Alkali Salt in Zebrafish LI Sidi, YE Zuguang, ZENG Xingyao, ZHOU Minggao, TAN Xiaowen, FAN Taiping, TAN Xinxin, SHENG Yihua Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 56-63.   DOI: 10.13438/j.cnki.jdzk.2022.05.010 Abstract （432）      PDF（pc） （8453KB）（84）       Knowledge map    Save To systematically analyze the gastrointestinal toxicity of dichroa alkali salt and explore the model animals suitable for such toxic drugs,the LC10 and MNLC of dichroa alkali salt in zebrafish were observed.The intestinal effects of dichroa alkali salt were evaluated by the zebrafish model,including evaluation of intestinal peristalsis inhibition,intestinal phenotype,and tissue pathology of intestinal tract.Results showed that the LC10 and MNLC of dichroa alkali salt on zebrafish were about 10 μg/mL and 5 μg/mL,respectively.Zebrafish were treated by dichroa alkali salt with concentration of 0.5,1.67,5 and 10 μg/mL,and the intestinal contents of zebrafish increased significantly.The inhibition rates of intestinal peristalsis were 27.78%,61.11%,77.11% and 80.89%,respectively,which showed that all concentrations of dichroa alkali salt has obvious inhibitory effect on the intestinal peristalsis of zebra fish.The intestinal toxicity phenotype and histopathological observation showed that under the conditions of 0.5,1.67,5 and 10 μg/mL salt treatment,the intestinal folds were reduced or disappeared,the intestinal mucosa cells were denaturated,the intestinal mucosa was destroyed,the intestinal lumen was blurred,and the intestinal lumen was narrowed,and the severity was positively correlated with the incidence.Above all,dichroa alkali salt presents definite gastrointestinal toxicity.Zebrafish can be used as a model animal to systematically study the toxic effects and characteristics of gastrointestinal toxic drugs. Reference | Related Articles | Metrics | Comments（0） Select Investigation of the Mechanism of Coptis chinensis for the Treatment of COVID-19 Based on Network Pharmacology and Molecular Docking FENG Bijun Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 64-73.   DOI: 10.13438/j.cnki.jdzk.2022.05.011 Abstract （568）      PDF（pc） （10961KB）（117）       Knowledge map    Save Based on network pharmacology and molecular docking,the mechanism of coptis chinensis for the treatment of COVID-19 has been investigated.The Chinese herbal medicine systems pharmacology platform (TCMSP) was used to screen the active components and targets of Coptis chinensis.GeneCards and OMIM screened the disease targets of COVID-19,R screened the common targets of drugs and diseases,and Cytoscape constructed a "drug-active ingredient- target-disease” interaction network,STRING constructed a common target protein interaction network,ClueGo performed gene function analysis for common targets,and R performed Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis for common targets.Then molecular docking was performed between the core components of Coptis chinensis active components and the main targets for the treatment of COVID-19.The preliminary exploration obtained 14 active ingredients of Coptis chinensis with 235 targets and 341 genes related to COVID-19.The key targets of Coptis chinensis in the treatment of COVID-19 include VEGFA,IL6,POR,and PLAT.Molecular docking results showed that the core compound of Coptis chinensis has a certain affinity for VEGFA,IL6,POR,and PLAT. Reference | Related Articles | Metrics | Comments（0） Select Study on the Mechanism of Lily in the Treatment of Cognitive Impairment Based on Network Pharmacology and Molecular Docking LIU Ye, FU Chuanjun, LIU Na, LI Xianhui, LIU Xihan Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 74-82.   DOI: 10.13438/j.cnki.jdzk.2022.05.012 Abstract （373）      PDF（pc） （8180KB）（236）       Knowledge map    Save The mechanism of lily in the treatment of cognitive impairment was studied based on network pharmacology and molecular docking.The main active components and action targets of lily were obtained from TCMSP database.After screening,7 main active components were obtained.There were 465 potential action targets of the main active components of lily.Secondly,disease targets were obtained from GeneCards,OMIM,PharmGKB,DrugBank and CTD databases,and the intersection of lily chemical components and cognitive impairment targets was obtained by using Venny software.44 drug component-disease intersection targets were obtained.Using STRING database,the intersection target protein interaction (PPI) network was constructed.After screening,the core targets and core components were obtained.It was found that MAOB (monoamine oxidase B),CASP3 caspase 3),ESR1 (estrogen receptor),JUN (gene of chromosome 1),CASP8 (aspartic acid proteolytic enzyme 8),CASP9 (aspartic acid proteolytic enzyme 9),PGR (steroid receptor gene),AR (adrenergic receptor),SLC6A3 (dopamine transporter gene),PTGS2 (cyclooxygenase-2) were the key target proteins of lily pharmacodynamic components for the anti cognitive impairment effect.Metascape and DAVID platforms were used for GO function enrichment and KEGG pathway enrichment analysis,and Autodock Vina platform was used for molecular docking.The results showed that 4 active compounds of lily were related to 10 key core targets of cognitive impairment,and there were 30 key signal pathways involved in the regulation.Among them,stigmasterol,3-norcolchicine,β-Sitosterol,and rosin acid had high degree value.The target proteins with high degree value included MAOB,PTGS2,CASP3,CASP9,etc.The main pathways involved were neural activity ligand receptor interaction pathway,calcium signaling pathway,small cell lung cancer pathway and so on.The mechanism of lily in treating cognitive impairment may improve cognitive function by inhibiting the activity of monoamine oxidase B. 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