Journal of Jishou University(Natural Sciences Edition) ›› 2022, Vol. 43 ›› Issue (5): 56-63.DOI: 10.13438/j.cnki.jdzk.2022.05.010

• Pharmacological mechanism • Previous Articles     Next Articles

Research on Gastrointestinal Toxicity Evaluation of Dichroa Alkali Salt in Zebrafish

LI Sidi,YE Zuguang,ZENG Xingyao,ZHOU Minggao,TAN Xiaowen,FAN Taiping,TAN Xinxin,SHENG Yihua   

  1. (1.College of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China;2.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;3.Xiangxi Minzu Hospital of Traditional Chinese Medicine,Jishou 416000,Hunan China;4.School of Medicine,Jishou University,Jishou 416000,Hunan China)
  • Online:2022-09-25 Published:2023-01-11

Abstract: To systematically analyze the gastrointestinal toxicity of dichroa alkali salt and explore the model animals suitable for such toxic drugs,the LC10 and MNLC of dichroa alkali salt in zebrafish were observed.The intestinal effects of dichroa alkali salt were evaluated by the zebrafish model,including evaluation of intestinal peristalsis inhibition,intestinal phenotype,and tissue pathology of intestinal tract.Results showed that the LC10 and MNLC of dichroa alkali salt on zebrafish were about 10 μg/mL and 5 μg/mL,respectively.Zebrafish were treated by dichroa alkali salt with concentration of 0.5,1.67,5 and 10 μg/mL,and the intestinal contents of zebrafish increased significantly.The inhibition rates of intestinal peristalsis were 27.78%,61.11%,77.11% and 80.89%,respectively,which showed that all concentrations of dichroa alkali salt has obvious inhibitory effect on the intestinal peristalsis of zebra fish.The intestinal toxicity phenotype and histopathological observation showed that under the conditions of 0.5,1.67,5 and 10 μg/mL salt treatment,the intestinal folds were reduced or disappeared,the intestinal mucosa cells were denaturated,the intestinal mucosa was destroyed,the intestinal lumen was blurred,and the intestinal lumen was narrowed,and the severity was positively correlated with the incidence.Above all,dichroa alkali salt presents definite gastrointestinal toxicity.Zebrafish can be used as a model animal to systematically study the toxic effects and characteristics of gastrointestinal toxic drugs.

Key words: dichroa alkali salt, gastrointestinal, toxicity, zebrafish

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