Effects of Astragaloside on Cardiomyocyte Apoptosis in Adriamycin-Induced Cardiomyopathy in Rats

Abstract

Abstract: Objective To explore the effects of Astragaloside on cardiomyocyte apoptosis and the expression of telomerase in adriamycin (ADR)-induced cardiomyopathy in rats.Methods Male SD rats weighing about 250 g were used to establish the model of Adriamycin-induced Cardiomyopathy，then randomized into groups:Astragaloside group，ADR group，control group and control+Astragaloside group.Apoptotic cardiomyocytes were detected using the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method (TUNEL).The expression of telomerase was determined by TRAP-PCR-ELISA method.Results Compared with control group，ADR group had significantly higher index of apoptotic cardiomyocytes (P<0.05).The apoptotic index in Astragaloside group was less than that in ADR group (P<0.05)，however significantly higher than that in control group and control+Astragaloside group (P<0.05).The expression of telomerase in Astragaloside group was significantly higher than that in ADR group(P<0.05)，however significantly lower than that in control group and control+Astragaloside group (P<0.05).Conclusions Myocardial apoptosis is an important mechanism of adriamycin-induced cardiomyopathy.Astragaloside therapy can inhibit cardiomyocyte apoptosis in adriamycin-induced cardiomyopathy，partly because it might increase expression of telomerase.

Key words: astragaloside, adriamycin, myocardium, apoptosis, telomerase

ZHONG Fei, LI Hui, ZHOU Wei-Hua. Effects of Astragaloside on Cardiomyocyte Apoptosis in Adriamycin-Induced Cardiomyopathy in Rats[J]. journal6, 2008, 29(6): 104-106.

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