Orderliness of Genes Expression Profiling in Viral Heart Disease

Abstract

Abstract: Objective To obtain the data on dynamic gene expression changes during the progression from myocarditis to dilated cardiomyopathy.Methods Four-weeks-old-male Balb/c mice were inoculated repetitively once month with Nancy strain of CVB.The gene expression profiles were investigated in mice cardiac tissue samples of acute and chronic viral myocarditis and dilated cardiomyopathy by cDNA microarry techniques,which include 8000 cDNA clones of mice,at days 0,7,21 and 3,6,9 months postinfection time points as compared with no-infection.A self-orangizing map(SOM) was used to analyze the data of differentially expressed genes with the GeneMaths software.Results (1) gene expressions were found up-regulated only at 7 day(cluster 10,14,15,19,20),21 day(cluster 21,22) or 7 and 21 days(cluster 23,24) respectively;(2) gene expressions down-regulated were found only at 7 (cluster 6,7,11,12) 21 day(cluster 4,5),or at 7 and 21 days(cluster 2,3,8);(3) genes expressions down-regulated were also demonstrated at 7 day and up-regulated at 21 day and 3 month( cluster 1,16).(4)some genes persisted up- (e.g.latent transforming growth factor beta binding protein,beta-2 microglobulin,CD 53 antigen) or down-regulated (e.g.alpha 1 microglobulin,ninjurin 1,kallikrein 27,cytokine inducible SH2-containing protein 2) through each points.Conclusion We have established dynamic gene expression profiling database during the progression from myocarditis to dilated cardiomyopathy in mice with CVB infection.The results provide new insight into potential molecular mechanisms of progression from myocarditis to dilated cardiomyopathy with CVB infection.

Key words: myocarditis, cardiomyopathy, cDNA microarry, Coxsackievirus B

LI Shuang-Jie, ZHONG Fei, ZHANG Zhao-Cai, CHEN Rui-Zhen, YANG Ying-Zhen, CHEN Hao-Zhu, GE Jun-Bo. Orderliness of Genes Expression Profiling in Viral Heart Disease[J]. journal6, 2008, 29(6): 107-111.

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