Optimization of Extraction Process and Vitro Antioxidant Activity of Total Polysaccharides from Polygonatum odoratum

doi:10.13438/j.cnki.jdzk.2024.06.006

Abstract

Abstract: The extraction process and antioxidant activity of total polysaccharide from the rhizome of Polygonatum odoratum were evaluated.The total polysaccharide was isolated from Polygonatum odoratum rhizome through ultrasound-assisted water bath heating extraction method.The influences of material-liquid ratio,ultrasonic power,ultrasonic extraction temperature on the extraction rate of the total polysaccharide were investigated.Based on the results of single-factor experiments,the response surface experiments give the optimal extraction conditions of total polysaccharide from the rhizome of Polygonatum odoratum∶ material-liquid ratio 1∶15 (g∶mL),ultrasonic power 300 W,extraction time 60 min,and ultrasonic extraction temperature 60 ℃.Under these extraction conditions,the total polysaccharide yield of Polygonatum odoratum was 13.58%.The results of the antioxidant activity test showed that when the mass concentration of Polygonatum odoratum polysaccharide was 2.75 g/L,the scavenging rates of Polygonatum odoratum polysaccharide on DPPH radicals and OH radicals were 34.18% and 62.04%,respectively.

Key words: Polygonatum odoratum, polysaccharide, extraction process, antioxidant activity, response surface method

ZHANG Yan, ZHANG Yang, CHEN Jiarui, YAO Shufeng, CHENG Jiang. Optimization of Extraction Process and Vitro Antioxidant Activity of Total Polysaccharides from Polygonatum odoratum[J]. Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 37-43.

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