Extraction of Bletilla striata Polysaccharides and Detection of its Antibacterial Activity

doi:10.13438/j.cnki.jdzk.2022.05.006

Abstract

Abstract: Bletilla striata polysaccharides were extracted by water extraction and alcohol precipitation,with different ratio of material to liquid,extraction temperature and extraction time as the research conditions and the extraction content as the index of orthogonal experiment,so as to determine the best extraction technology.Under the optimum conditions,the extracted polysaccharides were added to the ointment to make Bletilla striata ointment.The inhibitory effects of Bletilla striata polysaccharide and Bletilla striata ointment on the growth of Escherichia coli and Staphylococcus aureus were determined by filter paper diffusion method.The results showed that the optimum extraction conditions were as follows:the ratio of material to liquid was 1:35 (g:mL),the extraction temperature was 80 ℃ and the extraction time was 4.0 h.Under these extraction conditions,the yield of polysaccharide was 25.97%.When the mass concentration of Bletilla striata polysaccharide reached 0.33 g/mL,it had inhibitory effect on Escherichia coli and Staphylococcus aureus.When the mass concentration was increased to 0.66 g/mL,the bacteriostatic effect of polysaccharide extract was stronger,and the bacteriostatic effect on Escherichia coli was better than that of commercial Yifubaiying bacteriostatic paste.The performance of Bletilla striata ointment was stable and the bacteriostatic effect against Escherichia coli was stronger than Staphylococcus aureus.

Key words: Bletilla striata, polysaccharide, orthogonal method, escherichia coli, staphylococcus aureus

LUO Lingli, KUANG Yongbin, YU Xi, ZHANG Tao, FENG Qiuju. Extraction of Bletilla striata Polysaccharides and Detection of its Antibacterial Activity[J]. Journal of Jishou University(Natural Sciences Edition), 2022, 43(5): 37-42.

References

[1] 李世琴,熊丽丹,何海伦,等.白芨的药理作用和临床研究进展[J].中国美容医学,2021,30(11):176-178.
[2] 孔伟华,徐建波,崔琦,等.白及化学成分、药理作用和白及多糖提取工艺的研究进展[J].中医药信息,2021,38(9):69-78.
[3] 陈姿燕.白芨源多糖的分离纯化、结构解析以及抗氧化活性研究[D].上海:上海交通大学,2020.
[4] 张兵,史亚,周芳美,等.白及提取物体外抗流感病毒药效及其机理研究[J].中药材,2017,40(12):2930-2935.
[5] GUO Jingjing,DAI Binling,CHEN Nipi,et al.The Anti-Staphylococcus aureus Activity of the Phenanthrene Fraction from Fibrous Roots of Bletilla striata[J].BMC Complementary and Alternative Medicine,2016,16(1):491.
[6] 俞杭苏,代斌玲,钱朝东,等.白及须根化学成分及其体外抗菌活性研究[J].中药材,2016,39(3):544-547.
[7] 陈向阳,宋武,檀小菲,等.白及须根多酚提取工艺优化及其抗氧化、酪氨酸酶抑制活性研究[J].天然产物研究与开发,2020,32(7):1134-1142.
[8] 孔令姗,俞苓,胡国胜,等.白芨多糖的分子量测定及其吸湿保湿性评价[J].日用化学工业,2015,45(2):94-98.
[9] 张智芳,林文庭,陈灿坤.植物多糖提取工艺的研究进展[J].海峡预防医学杂志,2008(3):18-20.
[10] 梅光明,郝强,张小军,等.酸提香菇多糖的分离纯化及结构鉴定[J].现代食品科技,2014,30(9):79-84.
[11] 王萱萱,刘春宇,谢贝昱,等.碱提甘蔗皮多糖提取工艺、初步结构及其对α-葡萄糖苷酶的抑制作用[J].中国农业科学,2021,54(12):2653-2665.
[12] 张晓静.硫酸蒽酮法测定半夏多糖的含量研究[J].中国医药指南,2012,10(19):120-121.
[13] 马世宏,金玲,揭邃,等.白芨-丹皮酚包合物在化妆品中的应用研究[J].日用化学品科学,2009,32(6):30-33.