Journal of Jishou University(Natural Sciences Edition) ›› 2022, Vol. 43 ›› Issue (5): 47-51.DOI: 10.13438/j.cnki.jdzk.2022.05.008

• Pharmaceutical analysis • Previous Articles     Next Articles

Determination of Sesamin in Compound Tongye Burn Oil by HPLC

QIN Aitao,YANG Dequan,HAN Xiangyun,XU Yuhui   

  1. (1.Food and Drug Inspection Institute,Xiangxi Tujia and Miao Autonomous Prefecture,Jishou 416000,Hunan China;2.China Hunan Hongcheng Pharmaceutical Co.LTD,Jishou 416000,Hunan China)
  • Online:2022-09-25 Published:2023-01-11

Abstract: A method for the determination of sesamin in compound tongye burn oil by high performance liquid chromatography (HPLC) was established.The chromatographic conditions were:tade-pak af-c18 column (250 mm×4.6 mm,5  μm);mobile phase:methanol water (80∶20);flow rate:0.8 mL/min;detection wavelength:287 nm;column temperature:35 ℃;injection volume:10  μL.The number of theoretical plates shall be calculated as sesamin and shall not be less than 5 000.The linearity of sesamin reference substance was good in the range of 0.020 18~0.403 6 g/L (R=1),the regression equation was Y=15 562X+ 9.37,the recovery of sesamin was 103.22%,and the precision RSD was 0.58%.The results show that the method is simple,rapid,accurate,sensitive and stable,and can be used for the content detection and quality control of sesamin in compound tongye burn oil.

Key words: compound tongye burn oil, sesamin, sesame oil, HPLC

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