journal6 ›› 2007, Vol. 28 ›› Issue (1): 95-97.

• Biological resources • Previous Articles     Next Articles

Optimal Choice for Reaction System in RAPD Analysis of Euryodendron Excelsum H.T.Chang


  1. (1.Guangdong Vocational & Technical College of Pharmaceutical Engineering,Guangzhou 510520,China;2.School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China)
  • Online:2007-01-25 Published:2012-06-19

Abstract: The DNA templates of Euryodendron excelsum H.T.Chang extracted from young leaf were amplified by RAPD.Effects of the content of DNA temlates,primers,dNTP,Mg2+ and Taq DNA polymerase on experimental results were tested and the optimal reaction system of RAPD for Euryodendron excelsum H.T.Chang was determined as follows:25 ng DNA template,2.0 mmol/L Mg2+,0.018 μmol/L primer,250 μmol/L dNTP,0.5 u Taq DNA polymerase in total 25 μL reaction volume.

Key words: Euryodendron excelsum H.T.Chang, RAPD, reaction system, optimal choice

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