Simultaneous Determination of Several Polyphenolic Acids in Eucommia Ulmodies by HPLC——Comparison of Microwave and Ultrasound-Assisted Extraction with Heat Reflux Extraction Techniques for the Extraction of Polyphenolic Acids

journal6 ›› 2007, Vol. 28 ›› Issue (1): 102-109.

• Chemistry and chemical engineering • Previous Articles Next Articles

Simultaneous Determination of Several Polyphenolic Acids in Eucommia Ulmodies by HPLC——Comparison of Microwave and Ultrasound-Assisted Extraction with Heat Reflux Extraction Techniques for the Extraction of Polyphenolic Acids

(1.Hunan Provincial Key Laboratory of Forest Product and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000, Hunan China;2.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China)

Online:2007-01-25 Published:2012-06-19
About author:LI Hui(1971-),male,born in Cili County,Hunan Province,China,doctor degree in analytical chemistry,major in analytical chemistry,with a technical post of deputy professor.
Supported by:
Supported by the National Science Foundation of China(20275010)

Abstract

Abstract: Microwave- and ultrasonics-assisted extraction (MAE and UAE) and heat reflux extraction (HRE) techniques were respectively used for simultaneous extraction of five polyphenolic acids,i.e. chlorogenic acid,caffeic acid,gallic acid,protocatechuic acid and vanillic acid,from Eucommia ulmodies.The influence of extraction time and solvent type on the extraction yields of compounds tested was investigated.The time that the highest extraction yields are obtained is 90 min and 2 h for UAE and HRE respectively;whereas MAE needs only 50 s.While water is shown to be a better solvent than methanol in MAE and HRE techniques for these polyphenolic acids,methanol produces a higher extraction yield than that obtained using water in UAE.The repeatabilities of the three extraction techniques were shown to be satisfactory.Due to the high extraction yields and the considerable saving of time,MAE was more effective than UAE and HRE techniques in the extraction of these polyphenolic acids from Eucommia ulmodies.

Key words: Eucommia ulmodies, polyphenolic acids, microwave-assisted extraction, ultrasonically assisted extraction, heat reflux extraction

LI Hui, ZHANG Chao-Hui, LI Ya-Nan, LEI Hua-Ping, LI Zhi-Ping, ZHANG Yong-Kang. Simultaneous Determination of Several Polyphenolic Acids in Eucommia Ulmodies by HPLC——Comparison of Microwave and Ultrasound-Assisted Extraction with Heat Reflux Extraction Techniques for the Extraction of Polyphenolic Acids[J]. journal6, 2007, 28(1): 102-109.

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Simultaneous Determination of Several Polyphenolic Acids in Eucommia Ulmodies by HPLC——Comparison of Microwave and Ultrasound-Assisted Extraction with Heat Reflux Extraction Techniques for the Extraction of Polyphenolic Acids

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