Abstract: Objective To establish a method for determination of Puerarin in xingmaitong.Method HPLC was used,the separation was performed on Luna  C18 (5 μg,250 mm×4.6 mm)  column，a  mixture of  methanol－water（25∶75） was servered  as the mobile phase，and the  detecting wavelength was at 2 250 nm.Results The linearity range was from 4.16～83.2 mg/L，and the mean recovery was 100.84％，RSD 1.06％.Conclusion The method is simple,accurate and good for the determination of Puerarin in Xingmaitong,and it can be used for quality control for Xingmaitong.

Key words: Xingmaitong drop pills, puerarin, HPLC