journal6 ›› 2004, Vol. 25 ›› Issue (4): 35-38.

• Doctoral Forum • Previous Articles     Next Articles

Research on Genetic Transformation of Bacillus Brevis


  1. (1.College of Biological Resources and Environmental Science,Jishou University,Jishou 416000,Hunan China;2.Institute of Bio-engineering,Academy of Military Medical Science,Beijing 100071,China)
  • Online:2004-12-15 Published:2012-09-22

Abstract: Tris-polyethylene glycol(PEG) method,electroporation,and TTS were carried out for introducing plasmid DNA into 5 strains of wild Bacillus brevis.pBKE50 could be transformed into Bacillus brevis 50 and 735 by Tris-polyethylene glycol (PEG) method,and incorporated into Bacillus brevis 50 by electroporation.a transformation frequency is 102 transformants/μg DNA.

Key words: Bacillus brevis, Tris-polyethylene glycol (PEG) method, electroporation

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