Study on the Separation and Distribution ofα-amanitin in Amanita virosa

Abstract

Abstract: The compound α-amanitin from Amanita virosa was separated and determined by reversed-phase high performance liquid chromatography and UV-spectrum,by using 0.02　mol/L aqueous ammonium acetate-acetonitrile as mobile phase with gradient elution mode.The concentration and distribution of α-amanitin in A.virosa carpophores were studied,which were collected in SanJiang nature reserve of ABa zhou in SiChuan province.The results showed that α-amanitin aqueous solution was stable and the linear equation of α-amanitin quantitative analysis was Y=5　159x+1　097.4.The content of α-amanitin reached about 2　838.8　μg per gram dry fruit bodies and the distribution of α-amanitin was tissue dependent.The content of α-amanitin in gills was the highest,which was about 51.09% of total toxin per gram dry carpophores,and the concentration,the 0.29% of dry gills,was also the highest among the different tissues.

Key words: Amanita virosa, &alpha, -amanitin；RP-HPLC

LIU Jun, ZHANG Jie, CAO Mei, LI Da-Xu, YANG Zhi-Rong, ZHAO Jian. Study on the Separation and Distribution ofα-amanitin in Amanita virosa[J]. journal6, 2004, 25(4): 39-43.

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