Isolation,Purification and Properties of Chondroitinase From Serratia Marcescens

Abstract

Abstract: Chondroitinase was isolated and purified from Serratia marcescens and its kinetic property was examined.The Chondroitinase was purified by salting-out,DEAE-Sepharose FF ion-exchange chromatogram,and gel filtrition with Sephacryl S-200 chromatogram.The purified enzyme moved as a single electrophoretic band in PAGE.The specific activity was 81.23 U/mg.It’s subunit weight was 35 KD with SDS-PAGE,moleculer weight was 70.20 KD with gel filtrition on Sephacryl S-300(AKTA FPLC).The optimum pH value for the enzyme was 7.5.The optimum temperature for the enzyme was about 40 ℃.The Michealis-Menton constant(Km) was 3.98×10^-4mol/L with 4-Chondroitin Sulfate as its substrate.

Key words: Serratia marcescens, chondroitinase, isolation and purification

TAO Ke, LONG Zhang-Fu, LIU Kun, TAO Yong, JIN Hong, RAN Hong-Yan, GAO Qing, LIU Shi-Gui. Isolation,Purification and Properties of Chondroitinase From Serratia Marcescens[J]. journal6, 2004, 25(4): 44-48.

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