Journal of Jishou University(Natural Sciences Edition)

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Screening of High Efficiency Potash Feldspar-Solubilizing Bacteria by NTG/UV

PENG Yuyuan,YI Langbo,PENG Qingzhong,PENG Qingjing   

  1. (College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China)
  • Online:2016-11-25 Published:2016-12-13


In order to build a bacteria library with large difference genetic background and the Potassium-solubilizing performance,an original Silicate bacteria L18 (Bacillus cereus,KF494191) was selected.It was induced mutagenesis by nitrosoguanidine (NTG) culturing and ultraviolet (UV) radiating.42 mutant bacteria with obviousincreased potassium-solubilizing performance were screened from a large number of mutant bacteria.The K content in fermentation of 2 mutant bacteria (L18-NTG-151,L18-UV-68) were 126.74 mg/L and 121.69 mg/L,which were improved by 63.64% and 57.12% compared with original bacteria L18 (77.45 mg/L).The results show thatboth two methods of NTG or UV can significantly improve the potassium-solubilizing capacity of Bacillus cereus.

Key words: NTG, UV, mutation breeding, Potassium-solubilizing bacteria

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