Abstract: A temperature-sensitive imprinted polymer was prepared with diosgenin as the template and with α-methacrylic acid (MAA) and N-Isopropylacrylamide (NIPAM) as mixed-functional monomer in present work.Then,this imprint polymer was filled into an empty stainless steel tube to produce a molecularly imprinted polymer column and the retention and controlled release behavior for this column toward the template as a drug molecule were evaluated in chromatographic model.The influence of column temperature,effluent composition and pH,and effluent ion strength on the retention selectivity and controlled release behavior for this imprint column was investigated.Results indicated that column temperature possessed significant influence on retention and controlled-release behavior for the imprint column.When it was set at 303 K (near critical temperature of this imprinted polymer),the column had the strongest retention capacity toward the template.Moreover,when column temperature was higher and/or lower than 303 K,a decreased retention capacity and an accelerated template release for the imprint column was observed.In addition,effluent composition,pH and effluent ion strength also affect the retention and controlled-release for this imprint column.At 303 K column temperature and with effluent of  0.001 mol/L (pH 7.4),the release time was as long as 1 050 min.

Key words: molecular imprint, temperature-sensitive imprinted polymer, diosgenin, retention behavior, controlled-release of drug