Content of Pharmaceutical analysis in our journal

Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Thin Layer Chromatographic Identification of Cynanchum otophyllum Decoction Pieces and the Standard Limit of Extraction Inspection YANG Dequan, SHE Yonghong, JI Linfang, DING Ye Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 43-46.   DOI: 10.13438/j.cnki.jdzk.2022.05.007 Abstract （430）      PDF（pc） （2419KB）（88）       Knowledge map    Save A thin layer chromatographic identification method was established for decotion pieces of Cynanchum otophyllum.This method takes decotion pieces of Cynanchum otophyllum as the test sample,uses silica gel G thin layer plate,uses Cynanchum otophyllum as the control drug,uses ultrasonic treatment to prepare the test solution and the reference drug solution for 30 min,and uses cyclohexane ether butyl acetate glacial acetic acid (20∶6∶3∶0.2) as the developing agent.Under ultraviolet light (365 nm),the results showed that the chromatograms of 15 batches of decotion pieces of Cynanchum otophyllum were consistent with the chromatograms of Cynanchum otophyllum reference materials.This thin layer chromatographic method is stable and simple,and can be used to identify decotion pieces of Cynanchum otophyllum.According to the cold leaching method specified in the Chinese Pharmacopoeia (2020),the water-soluble extracts of 15 batches of decotion pieces of Cynanchum otophyllum were 15.55%~22.96%.Therefore,it is recommended that the limit of detection of the extracts of decotion pieces of Cynanchum otophyllum should be "not less than 15.0%". Reference | Related Articles | Metrics | Comments（0） Select Determination of Sesamin in Compound Tongye Burn Oil by HPLC QIN Aitao, YANG Dequan, HAN Xiangyun, XU Yuhui Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 47-51.   DOI: 10.13438/j.cnki.jdzk.2022.05.008 Abstract （324）      PDF（pc） （533KB）（66）       Knowledge map    Save A method for the determination of sesamin in compound tongye burn oil by high performance liquid chromatography (HPLC) was established.The chromatographic conditions were:tade-pak af-c18 column (250 mm×4.6 mm，5  μm)；mobile phase:methanol water (80∶20);flow rate:0.8 mL/min;detection wavelength:287 nm;column temperature:35 ℃;injection volume:10  μL.The number of theoretical plates shall be calculated as sesamin and shall not be less than 5 000.The linearity of sesamin reference substance was good in the range of 0.020 18~0.403 6 g/L (R=1),the regression equation was Y=15 562X+ 9.37,the recovery of sesamin was 103.22%,and the precision RSD was 0.58%.The results show that the method is simple,rapid,accurate,sensitive and stable,and can be used for the content detection and quality control of sesamin in compound tongye burn oil. Reference | Related Articles | Metrics | Comments（0） Select Simultaneous TLC Identification of Paulownia Leaf and Sesame Oil in Compound Tongye Burn Oil HAN Xiangyun, YANG Dequan, QIN Aitao, XU Yuhui Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 52-55.   DOI: 10.13438/j.cnki.jdzk.2022.05.009 Abstract （377）      PDF（pc） （2683KB）（83）       Knowledge map    Save Qualitative identification of paulownia leaf and sjianyesame oil in compound Tongye burn oil was carried out by thin layer chromatography with medicinal tung leaf,ursolic acid and sesamin as reference materials.Silica gel G thin layer plate was used.The developer was cyclohexane ether butyl acetate glacial acetic acid (20∶5∶4∶0.2).The results showed that all spots in the solution chromatography of the test sample were separated clearly,ursolic acid Rf=0.45,sesamin Rf==0.23,Spots of the same color are displayed on the corresponding positions of the reference medicine and the reference substance.This method is simple and reproducible,and can be used for the quality control of compound Tongye burn oil. Reference | Related Articles | Metrics | Comments（0）