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    Analysis of the Coagulant Activity and Chemical Components in Artemisia annua L.
    ZHAO Fafa, ZHANG Xiaorong
    Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 30-36.   DOI: 10.13438/j.cnki.jdzk.2022.05.005
    Abstract543)      PDF(pc) (2305KB)(132)       Save
    The pharmacodynamics substance in Artemisia annua L.was studied.The hemostasis chemical compositions were extracted by the organic solvent extraction method in Artemisia annua L..The hemostasis medicinal effect of the extract from Artemisia annua L.was detected using the coagulation plate method,the test tube method and the plasma recalcification time measurement method.The flavone compounds were separated from the butyl alcohol extract using Alkali dissolution-acid precipitation method.The spectroscopic properties were detected by the ultraviolet-visible spectroscopy and the fluorescence spectroscopy.And the flavone compounds were identified of the physical and chemical properties.The terpenoids compounds were separated from the butyl alcohol extract by the low temperature method,and were analyzed using the thin layer chromatography (TLC).The results indicated that the butyl alcohol extract was the hemostasis pharmacodynamics substance in Artemisia annua L..The clotting effect was significant of the butyl alcohol extract tested by the three methods of the coagulation plate method,the test tube method and the plasma recalcification time measurement method.And the chemical composition analysis showed that a lot of flavone compounds and the terpenoids compounds were contained in the butyl alcohol extract.It can be concluded that the pharmacodynamics substance is the butyl alcohol extract from Artemisia annua L.,and the major chemical constituents include the flavone compounds and the terpenoids compounds.
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    Extraction of Bletilla striata Polysaccharides and Detection of its Antibacterial Activity
    LUO Lingli, KUANG Yongbin, YU Xi, ZHANG Tao, FENG Qiuju
    Journal of Jishou University(Natural Sciences Edition)    2022, 43 (5): 37-42.   DOI: 10.13438/j.cnki.jdzk.2022.05.006
    Abstract441)      PDF(pc) (2796KB)(108)       Save
    Bletilla striata polysaccharides were extracted by water extraction and alcohol precipitation,with different ratio of material to liquid,extraction temperature and extraction time as the research conditions and the extraction content as the index of orthogonal experiment,so as to determine the best extraction technology.Under the optimum conditions,the extracted polysaccharides were added to the ointment to make Bletilla striata ointment.The inhibitory effects of Bletilla striata polysaccharide and Bletilla striata ointment on the growth of Escherichia coli and Staphylococcus aureus were determined by filter paper diffusion method.The results showed that the optimum extraction conditions were as follows:the ratio of material to liquid was 1:35 (g:mL),the extraction temperature was 80 ℃ and the extraction time was 4.0 h.Under these extraction conditions,the yield of polysaccharide was 25.97%.When the mass concentration of Bletilla striata polysaccharide reached 0.33 g/mL,it had inhibitory effect on Escherichia coli and Staphylococcus aureus.When the mass concentration was increased to 0.66 g/mL,the bacteriostatic effect of polysaccharide extract was stronger,and the bacteriostatic effect on Escherichia coli was better than that of commercial Yifubaiying bacteriostatic paste.The performance of Bletilla striata ointment was stable and the bacteriostatic effect against Escherichia coli was stronger than Staphylococcus aureus.
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