Abstract: OBJECTIVE To establish a RP－HPLC method for the determination of Shikimic Acid in Illicium verum Hook.f.METHODS In RP－HPLC analysis,a Waters Symmetry C18 column (5 μm,4.6 mm×150 mm,i.d.) was selected as separation column at 30 ℃±1 ℃.The mobile phase consisted of 0.01 mol/L K2HPO4 phosphate buffer (pH=3,I =0.17 mol/L) and 3% methanol.The flow rate was 0.5 mL/min and the wavelength of measurement was 220 nm.The average recovery was 97.76% with RSD of 1.937% (n=5).CONCLUSION The method was proved to be quick,simple,and highly sensitive and can be useful in Shikimic Acid medicines quality control.

Key words: RP－HPLC, Illicium verum Hook.f., Shikimic Acid