journal6 ›› 2008, Vol. 29 ›› Issue (1): 104-106.

• Chemistry and chemical engineering • Previous Articles     Next Articles

Reversed-Phase HPLC Determination of Shikimic Acid in Illicium Verum Hook.F.


  1. (1.College of Biology and Environmental Science,Jishou University,Jishou,Hunan 416000,China;2.Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Zhangjiajie Campus of Jishou University,Zhangjiajie Hunan 427000,China;3.College of Food Science,South China Agriculture University,Guang zhou 510642,China)
  • Online:2008-01-25 Published:2012-05-26

Abstract: OBJECTIVE To establish a RP-HPLC method for the determination of Shikimic Acid in Illicium verum Hook.f.METHODS In RP-HPLC analysis,a Waters Symmetry C18 column (5 μm,4.6 mm×150 mm,i.d.) was selected as separation column at 30 ℃±1 ℃.The mobile phase consisted of 0.01 mol/L K2HPO4 phosphate buffer (pH=3,I =0.17 mol/L) and 3% methanol.The flow rate was 0.5 mL/min and the wavelength of measurement was 220 nm.The average recovery was 97.76% with RSD of 1.937% (n=5).CONCLUSION The method was proved to be quick,simple,and highly sensitive and can be useful in Shikimic Acid medicines quality control.

Key words: RP-HPLC, Illicium verum Hook.f., Shikimic Acid

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