journal6 ›› 2008, Vol. 29 ›› Issue (4): 75-82.

• Chemistry and chemical engineering • Previous Articles     Next Articles

Analogue Recognition and Chromatographic Behaviors of MolecularlyImprinted Polymer for Protocatechuic Acid


  1. (1.Hunan Province Key Laboratory of Forstry Products & Chemical Engineering,Jishou University,Zhangjiajie 427000,Hunan China;2.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China)
  • Online:2008-07-25 Published:2012-05-21
  • About author:Correspondence author,LI Hui(1971-),male,was born in Cili,Hunan,master,associate professor;research area is the new analytical method in
  • Supported by:

    Science Research Foundation of Administration of Education of Hunan Province

Abstract:  A non-covalent imprinting approach was applied to synthesize protocatechuic acid (3,4-dihydroxybenzoic acid) imprinted polymers (MIPs) in the polar tetrahydrofuran by using methacrylic acid as functional monomer.By equilibrium adsorption experiments and high performance liquid chromatography (HPLC),the rebinding properties of the MIPs to protocatechuic acid (template) and its analogues were evaluated.It was found that the MIPs exhibited high adsorption capability and selectivity for the template and two kinds of absorption sites in the polymer were detected by Scatchard curve analysis.The eluents and the mobile phase flow rate had an important influence on the retention of the analytes on the MIPs column.All of the results showed that the stronger affinity of the MIPs to the template than its analogues resulted from the size and shape recognition in the MIPs network based on hydrogen bonding interaction.

Key words: molecularly imprinted polymer, protocatechuic acid, equilibrium adsorption, HPLC

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