Abstract: Soluble protein antigens from different developmental stages of Periplaneta fuliginosa were used to immunize rabbits.The immunological characteristics of antigens from different stages of Periplaneta fuliginosa were analyzed with SDS-PAGE and enzyme-linked immuno-blotting (ELIB).Rabbits anti-sera against nymph and adult antigens were used to screen the cDNA library of Periplaneta Americana nymph,and the positive clones were identified by PCR.Results showed that the egg antigens,nympha antigens,male and female adult antigens revealed 13,28,26,41 bands,respectively.The results also showed that there were cross-reactive antigen components among these groups.One and 6 new genes were screened with nympha and adult antigen immunized rabbit sera,respectively.

Key words: Periplaneta fuliginosa, imunized rabbit serum, preparation, ELIB, cDNA library screening