Abstract: Objective To explore whether  the method of determination of the total sugar in enzyme solution of eucommial seed shell can be feasible.Mothods The total sugar content was taken as the study indicators of digestion rate.In the same conditions，eucommial seed shell was degraded by the same amount of different types of enzymes and by the different amounts of an enzyme respectively.When sample was taken as blank fiduciary the total sugar was determined with anthrone colorimetry.Results The measured  total sugar of the enzyme solution better reflected the different rates of digestion in the 625 nm wavelength,and the total sugar determined with anthrone colorimetry and total sugar content as an indicator of the enzymes rate will not be affected by enzyme in enzyme solution of eucommial seed shell.Conclusions The method can eliminate interfering factor of the enzyme solution with differential spectrophotometry (△A method),which can prove a simple and accurate method for an indicator of the enzymes rate in the pretreatment samples of raw materials.

Key words: shell of eucommia ulmoide, total sugar content, anthrone colorimetry, enzymatic method, investigation targets；differential spectrophotometry