Abstract: In order to obtain sterile materails from Huperzia serrata,H2O2,C2H5OH,and HgCl2 were used to disinfect the explants,and the solutions of antibiotic compound together with hot water treatments were used to eliminate the endophytes.The results were as follows:the optimum method of the sterilization of surface and elimination of endophytes for stem was obtained by soaking the explants in hot water (47 ℃) for 1 h,followed by 70% C2H5OH for 30 s and then by 0.1 % HgCl2 for 7 min.The optimum method of the sterilization of surface for sporangium and stem apex was obtained by soaking the explants in 70 % C2H5OH for 30 s,followed by 0.1% HgCl2 for 4 min.Disinfectants together with hot water treatment can obviously reduce the pollution rate of explants.Sporangium and stem apex,which barely contain endophytes,sre excellent explants for plant tissue culture.

Key words: Huperzia serrata, explants, sterilization, endophytes