Characterization and Phylogenetic Analysis of the Chloroplast Genome of the Toxic Medicinal Plant Illicium simonsii

doi:10.13438/j.cnki.jdzk.2025.03.008

Abstract

Abstract: In order to explore the chloroplast genome characteristics of the toxic medicinal plant Illicium simonsii and analyze its relationships with other species of the Illicium,this study used next-generation sequencing (NGS) technology to perform high-throughput sequencing for leaf material of Illicium simonsii,and obtained the complete chloroplast genome after assembly and annotation,which was analyzed using bioinformatics.Meanwhile,phylogenetic tree of Illicium simonsii and the related species in the Illicium genus were constructed based on chloroplast genomes and coding sequences (CDS).The result showed that the full length of the Illicium simonsii chloroplast genome was 143 012 bp,which encoded 112 genes,including 78 protein-coding genes,29 tRNA genes,4 rRNA genes,and 1 pseudogene,with a total GC molar fraction of 39.1%;there were a total of 100 simple sequence repeats (SSRs) in the chloroplast genome of Illicium simonsii,with the largest number of mono-nucleotides.The degree of chloroplast genomic variation in Illicium plants all showed higher LSC and SSC regions than IRs regions,and the five highly variable regions were trnK-UUU-rps16，accD-psal，rpl20-clpP1，ycf1，atpF;Within the genus Illicium,Illicium simonsii was most closely related to Illicium burmanicum,with a support rate as high as 100%,and both Illicium simonsii and Illicium verum belong to the same monophyletic clade,exhibiting closer phylogenetic affinity.

Key words: Illicium simonsii, Illicium, complete chloroplast genome, phylogeny, genetic relationship

GAO Tiantian, MAO Jiuyang, LI Haifeng, ZHANG Dequan, 3. Characterization and Phylogenetic Analysis of the Chloroplast Genome of the Toxic Medicinal Plant Illicium simonsii[J]. Journal of Jishou University(Natural Sciences Edition), 2025, 46(3): 62-74.

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