journal6 ›› 2014, Vol. 35 ›› Issue (2): 85-89.DOI: 10.3969/j.issn.1007-2985.2014.02.019

• Biological resources • Previous Articles     Next Articles

Phylogenetic Analysis of Three Wild Grapes Based on PsbA-trnH,RbcL and trnL-F Sequences

 TANG  Yun-Tao, SU  Gui-Rong, XIE  Yu, YI  Lang-Bo   

  1. (1.College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China.2.Key Laboratory of Plant Resource Conservation and Utilization,Jishou University,Jishou 416000,Hunan China)
  • Online:2014-03-25 Published:2014-05-09

Abstract: PsbA-trnH,RbcL and trnL-F fragments were amplified from the three wild grapes in Xiangxi Autonomous Prefecture by PCR,and cloned into pMD18-T vector for sequencing.Based on PsbA-trnH,RbcL and trnL-F gene sequences,the phylogenetic trees between these three wild grapes and other related Vitaceae plants were constructed by software Mega4 for analyzing their phylogenetic relationship.The results showed that all of these three cpDNA fragments could be used as molecular markers in phylogenetic analysis among genera of Vitaceae,but of which results had a little difference from that of traditional morphological methods.Furthermore,RbcL gene fragment could be used for molecular identification among Vitis species.

Key words: wild grape, PsbA-trnH, RbcL, trnL-F, phylogeny

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