Abstract: Objective To develop a rapid,sensitive and specific real-time reverse transcription PCR(RT-PCR) for detecting respiratory syncytial virus (RSV).Method The primer and probe were designed according to the RSV gene sequency,and the real-time reverse transcription PCR reaction was optimized.The real time RT-PCR was applied to detect 686 clinical respiratory specimens.Results  The RSV was detected by real time RT-PCR accurately and quickly.Among those 686 specimens,113(16.5%) were positive for RSV.Conclusion Real time RT-PCR is a reliable and accurate assay for RSV.The detecting method can be applied to the clinical diagnosis of the RSV infection in children.

Key words: real time reverse transcription PCR, respiratory virus