Abstract: Objective： to study the influence of viola diffusa gine (VDG) extract on myocardial cells.Methods:myocardial cells were cultured with different concentrations of VDG extract to intervene.The myocardial cells pulse frequency and the contents of lactate dehydrgenase(LDH) in culture solution were determined.MTT method was used to observe the cells survival rate.Results:free radicals injured myocardial cell,VDG extract reduced LDH leakage and increased the cell survival rate (P<0.05).Conclusion:The results indicate that VDG extract has a certain protective effects on myocardial cells injured by free radicals.

Key words: Viola diffusa Ging(VDG), myocardial cells, free radicals