journal6 ›› 2000, Vol. 21 ›› Issue (3): 67-69.

• Chemistry and chemical engineering • Previous Articles     Next Articles

Purification and Determination of Molecular Size of the Plasmid pATZ-1


  1. ( 1. Department of Biology, College of Education, Tianjin Normal University, Tianjin 300020, China;2. Institute of Molecular Biology, Nankai University , Tianjin 300071, China)
  • Online:2000-09-15 Published:2013-01-16

Abstract: The plasmid pATZ- 1 is isolated and purified from Pseudomonas sp. strain AD1 that degrades herbicide atrazine by CsCl- EB density gradient centrifugation. pATZ- 1 is digested by EcoRI and produced 14 restraction fragments. The sum total of the sizes of 14 restriction fragments, i. e. the molecular size of pATZ- 1, is 125 kb.

Key words: atrazine, biodegradation, Pseudomonas AD1, plasmid pATZ-1, molecular size

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