journal6 ›› 2011, Vol. 32 ›› Issue (4): 88-91.

• Biological resources • Previous Articles     Next Articles

Prokaryotic Expression and Detection of Cyclohexanone Monooxygenase from Rhodococcus ruber JDM312


  1. (College of Biology and Environmental Sciences,Jishou University,Jishou 41600,China)
  • Online:2011-07-25 Published:2012-04-07

Abstract: The chnB gene encoding a cyclohexanone monooxygenase was isolated from the recombinant plasmid pMD18-chnB by digested with BamHI and HindⅢ,then cloned into the expression vector pQE30 and expressed in E. coli BL21 by IPTG induction.The expressed protein was identified by SDS-PAGE.The sequence analyses showed that the coding region of the chnB of Rhodococcus ruber JDM312 was 1 623 bp in length,and the predicted primary protein was composed of 540 amino acids.The SDS-PAGE analyses revealed a single protein band with a molecular weight of 60 kDa,suggesting that the recombinant plasmid of pQE30-chnB was successfully constructed and the target protein was expressed in E. coli.

Key words: rhodococcus ruber, cyclohexanone monooxygenase, chnB gene, prokaryotic expression

WeChat e-book chaoxing Mobile QQ