Abstract: In this study,tissue culture was explored to solve the propagate problem in the production with Huperzia serrata(Thunb)Trev.The stem and shoot-tips were used as explants to induce callus.These explants are cultured in four culture mediums:(1) 1/2MS+0.05 μmol/L IBA+1.4μmol/L KT+2 mg/L pirydoxine;(2) MS+0.05 μmol/L IBA+1.4 μmol/L KT+2 mg/L pirydoxine;(3) 1/2MS+2 mg/L pirydoxine;(4) MS+2 mg/L pirydoxine.The results show that the suitable element compositions to induce callus is:MS+0.05 μmol/L IBA+1.4μmol/L KT+2 mg/L pirydoxine.During tissue culture,fungal pollution is very serious,so the surface disinfection is important.There are two variants of surface sterilization.In the first variant shoot-tips were soaked in 70% ethyl alcohol (C₂H₅OH) for 1 min,5% sodium hypochlorite (NaOCl) for 1 min and then in 7% hydrogen peroxide (H₂O₂) for 10 min;the second variant were soaked in HgCl for 3 min.The result shows that the most suitable procedure for surface sterilization of explants is the second one.

Key words: Huperzia serrata(Thun)Trev, tissue culture, callus