Progress in Proteomic Research of Plasma Membrane and its Microdomains

Abstract

Abstract: Plasma membrane proteomic has become the difficulty and hot spot of proteomic research for its low abundance and strong hydrophobicity.With mammal cytoplasma membrane as an example,this article introduces the progress in proteomic research of plasma membrane and its microdomains mainly from the following aspects:the structure,concentrates,dissolution,separation and identification for plasma membrane and its microdomain as well as complex of membrane protein.

Key words: plasma membrane, membrane microdomains, plasma membrane proteomics, membrane protein complex

DENG Huo-Bao, LI Xuan-Wen, LIU Wei, CHEN Ping. Progress in Proteomic Research of Plasma Membrane and its Microdomains[J]. journal6, 2008, 29(3): 97-104.

References

[1] YATES J R,GILCHRIST A,HOWELL K E,et al.Proteomics of Organelles and Large Cellular Structures [J].Nat Rev Mol Cell Biol,2005,6:702-714.

[2] AU C E,BELL A W,GILCHRIST A,et al.Organellar Proteomics to Create the Cell Map [J].Curr Opin Cell Biol,2007,19:376-385.

[3] HOPKINS A L,GROOM C R.The Druggable Genome [J].Nat Rev Drug Discov,2002(1):727-730.

[4] YAMADA E.The Fine Structure of Bladder Epithelium of the Mouse [J].J Biochem Cyto,1:445-458.

[5] CHEN P,LI X,SUN Y,et al.Proteomic Analysis of Rat Hippocampal Plasma Membrane:Characterization of Potential Neuronal-Specific Plasma Membrane Proteins [J].J Neurochem,2006,98:1 126-1 140.

[6] ZHANG L,WANG X,PENG X,et al.Immunoaffinity Purification of Plasma Membrane with Secondary Antibody Superparamagnetic Beads for Proteomic Analysis [J].J Proteome Res,2007(6):34-43.

[7] CAO R,LI X,LIU Z,et al.Integration of a Two-Phase Partition Method into Proteomics Research on Rat Liver Plasma Membrane Proteins [J].J Proteome Res,2006(5):634-642.

[8] MORRE D J,MORRE D M.Preparation of Mammalian Plasma Membranes by Aqueous Two-Phase Partition [J].Biotechniques,1989(7):946-948,950-954,956-958.

[9] PERSSON A,JERGIL B.The Purification of Membranes by Affinity Partitioning [J].Faseb J,1995(9):1 304-1 310.

[10] EKBLAD L,JERGIL B,GIEROW J P.Purification of Rabbit Lacrimal Gland Plasma Membranes by Aqueous Two-Phase Affinity Partitioning [J].J Chromatogr B Biomed Sci Appl,2000,743:397-401.

[11] PERSSON A,JERGIL B.Purification of Plasma Membranes by Aqueous Two-Phase Affinity Partitioning [J].Anal Biochem,1992,204:131-136.

[12] ZHAO Y,ZHANG W,KHO Y,et al.Proteomic Analysis of Integral Plasma Membrane Proteins [J].Anal Chem,2004,76:1 817-1 823.

[13] OH P,SCHNITZER J E.Immunoisolation of Caveolae with High Affinity Antibody Binding to the Oligomeric Caveolin Cage：Toward Understanding the Basis of Purification [J].J Biol Chem,1999,274:23 144-23 154.

[14] FOSTER L J,DE HOOG C L,MANN M.Unbiased Quantitative Proteomics of Lipid Rafts Reveals High Specificity for Signaling Factors [J].Proc Natl Acad Sci U S A,2003,100:5 813-5 818.

[15] MCMAHON K A,ZHU M,KWON S W,et al.Detergent-free Caveolae Proteome Suggests an Interaction with ER and Mitochondria [J].Proteomics,2006(6):143-152.

[16] ZHANG L J,WANG X E,PENG X,et al.Proteomic Analysis of Low-Abundant Integral Plasma Membrane Proteins Based on Gels [J].Cell Mol Life Sci,2006,63:1 790-1 804.

[17] WASHBURN M P,WLOTERS D,YATES J R.Large-Scale Analysis of the Yeast Proteome by Multidimensional Protein Identification Technology [J].Nat Biotechnol,2001,19:242-247.

[18] JOSIC D,BROWN M K,HUANG F,et al.Use of Selective Extraction and Fast Chromatographic Separation Combined with Electrophoretic Methods for Mapping of Membrane Proteins [J].Electrophoresis,2005,26:2 809-2 822.

[19] DOMON B,AEBERSOLD R.Mass Spectrometry and Protein Analysis [J].Science,2006,312:212-217.

[20] KASHINO Y,HARAUAMA T,PAKRASI H B,et al.Preparation of Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis [J].J Chromatogr B Analyt Technol Biomed Life Sci,2007,849:282-92.

[21] BRAUN R J,KINKL N,BEER M,et al.Two-Dimensional Electrophoresis of Membrane Proteins [J].Anal Bioanal Chem,2007,389:1 033-1 045.

[22] BUREE J,BECKHAUS T,SCHAGGER H,et al.Analysis of the Synaptic Vesicle Proteome using Three Gel-Based Protein Separation Techniques [J].Proteomics,2006(6):6 250-6 262.

[23] WITTIG I,BRAUN H P,SCHAGGER H.Blue native PAGE [J].Nat Protoc,2006(1):418-428.

[24] REISINGER V,EICHACKER L A.Analysis of Membrane Protein Complexes by Blue Native PAGE [J].Proteomics,2006，6(2):6-15.

[25] BABUSIAK M,MAN P,PETRAK J,et al.Native Proteomic Analysis of Protein Complexes in Murine Intestinal Brush Border Membranes [J].Proteomics,2007(7):121-129.

[26] KATZ A,WARIDEL P,SHEVCHENKO A,et al.Salt-Induced Changes in the Plasma Membrane Proteome of the Halotolerant Alga Dunaliella salina as Revealed by Blue Native Gel Electrophoresis and Nano-LC-MS/MS Analysis [J].Mol Cell Proteomics,2007(6):1 459-1 472.

[27] PEIRCE M J,WAIT R,BEGUM S,et al.Expression Profiling of Lymphocyte Plasma Membrane Proteins [J].Mol Cell Proteomics,2004(3):56-65.

[28] DELOM F,SZPONARSKI W,SOMMERER N,et al.The Plasma Membrane Proteome of Saccharomyces Cerevisiae and Its Response to the Antifungal Calcofluor [J].Proteomics,2006(6):3 029-3 039.

[29] RODRIGUEZ-ORTEGA M J,NORAIS N,BENSI G,et al.Characterization and Identification of Vaccine Candidate Proteins Through Analysis of the Group A Streptococcus Surface Proteome [J].Nat Biotechnol,2006,24:191-197.

[30] ZHANG N,LI L.Effects of Common Surfactants on Protein Digestion and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis of the Digested Peptides Using Two-Layer Sample Preparation [J].Rapid Commun Mass Spectrom,2004,18:889-896.

[31] ZHOU J,ZHOU T,CAO R,et al.Evaluation of the Application of Sodium Deoxycholate to Proteomic Analysis of Rat Hippocampal Plasma Membrane [J].J Proteome Res,2006(5):2 547-2 553.

[32] LU X,ZHU H.Tube-Gel Digestion:A Novel Proteomic Approach for High Throughput Analysis of Membrane Proteins [J].Mol Cell Proteomics,2005(4):1 948-1 958.

[33] CAO R,HE Q,ZHOU J,et al.High-Throughput Analysis of Rat Liver Plasma Membrane Proteome by a Nonelectrophoretic in-Gel Tryptic Digestion Coupled with Mass Spectrometry Identification [J].J Proteome Res,2008(7):535-545.

[34] ZHONG H,MARCUS S L,LI L.Microwave-Assisted Acid Hydrolysis of Proteins Combined with Liquid Chromatography MALDI MS/MS for Protein Identification [J].J Am Soc Mass Spectrom,2005,16:471-481.

[35] BLONDER J,TERUNUMA A,CONRADS T P,et al.A Proteomic Characterization of the Plasma Membrane of Human Epidermis by High-Throughput Mass Spectrometry [J].J Invest Dermatol,2004,123:691-699.

[36] MCCLATCHY D B,LIAO L,PARK S K,et al.Quantification of the Synaptosomal Proteome of the Rat Cerebellum During Post-Natal Development [J].Genome Res,2007,17:1 378-1 388.

[37] SPEERS A E,BLACKLER A R,WU C C.Shotgun Analysis of Integral Membrane Proteins Facilitated by Elevated Temperature [J].Anal Chem,2007,79:4 613-4 620.

[38] GINGRAS A C,GSTAIGER M,RAUGHT B,et al.Analysis of Protein Complexes using Mass Spectrometry [J].Nat Rev Mol Cell Biol,2007(8):645-654.

[39] RINNER O,MUELLER L N,HUBALEK M,et al.An Integrated Mass Spectrometric and Computational Framework for the Analysis of Protein Interaction Networks [J].Nat Biotechnol,2007,25:345-352.

[40] Lockwich T,Pant J,Makusky A,et al.Analysis of TRPC3-Interacting Proteins by Tandem Mass Spectrometry [J].J Proteome Res,2008.

[41] SARDIU M E,CAI Y,JIN J,et al.Probabilistic Assembly of Human Protein Interaction Networks from Label-Free Quantitative Proteomics [J].Proc Natl Acad Sci U S A,2008,105:1 454-1 459.

[42] RYBAK J N,ROESLI C,KASPAR M,et al.The Extra-Domain A of Fibronectin is a Vascular Marker of Solid Tumors and Metastases [J].Cancer Res,2007,67:10 948-10 957.

[43] OH P,LI Y,YU J,et al.Subtractive Proteomic Mapping of the Endothelial Surface in Lung and Solid Tumours for Tissue-Specific Therapy [J].Nature,2004,429:629-635.