Abstract: Absorption and separation of the yellow pigment from Oenothera biennis L.by macroreticular resin were studied.Comparative studies of five resins（D-101，DA-201，DM-301，DS-401，X-5）showed that DS-401 resin had the best effect on absorption of the yellow pigment from Oenothera biennis L.Results of effects on desorption of different concentration ethanol and solvent with DS-401 resin as absorbent showed that acetone and ethylethamoate had good effect on desorption.

Key words: oenothera biennis L., yellow pigment, macroreticular resin, absorption and desorption